Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Zanthoxylum piperitum isolated culture process

A technology for in vitro culture and pepper wood, applied in the field of plant tissue culture and pepper wood in vitro culture, can solve problems such as inability to meet, and achieve the effects of good rooting effect, good market prospects, and high germination rate.

Inactive Publication Date: 2008-10-22
SHENZHEN POLYTECHNIC
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pepperwood is generally propagated by cuttings, but the cuttings are affected by the material of the mother plant and the breeding season, and often cannot meet the needs of the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Zanthoxylum piperitum isolated culture process
  • Zanthoxylum piperitum isolated culture process
  • Zanthoxylum piperitum isolated culture process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The method for in vitro culture of the pepper wood stem section provided by the specific embodiment comprises the following steps:

[0036] 1. Preparation of Explants

[0037] This step includes preparation and disinfection of explants. First of all, select the young shoots that grow vigorously in the current year, remove the leaves with sharp scissors, and then wash the branches with leaves removed with distilled water. Branches are then cut into small sections of 2 to 3 centimeters, noting that each section of explant should contain at least one lateral bud, and the branch containing lateral buds in each small section is the explant. The explants were rinsed and soaked in distilled water for a period of time, and then sterilized. First disinfect with 75% alcohol for 15 seconds, and then disinfect with 0.1% mercuric chloride solution for 3 minutes (add Tween-20). After the disinfection is completed, rinse with sterile water for 7 to 8 times before inoculation and su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an isolated culture method of zanthoxylum piperitum, comprising the steps such as preparing explant, induction, proliferation and rootage, etc. Preparing explant is to prepare stems of zanthoxylum piperitum which is fit for germination; the induction is to inoculate the explant to the medium for inducing plumule, thus causing the plumule to come up on the explant; proliferation is to inoculate the plumule to the proliferation medium for generating cluster buds; and rootage is to inoculate the cluster buds to the rootage medium, thus causing the cluster buds to take root and becoming the seedling of zanthoxylum piperitum. By adopting the technical proposal of the invention, as the tissue culture technology is adopted for isolated culture of zanthoxylum piperitum, the isolated culture method of zanthoxylum piperitum in the invention can not be restricted by seasons, a great deal of seedlings can be propagated rapidly at low cost, therefore, the economic benefit is considerable and the market prospect is good.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to plant tissue culture technology, in particular to a method for in vitro culture of pepper wood. Background technique [0002] Zanthoxylum Piperitum is a perennial evergreen shrub of Rutaceae, also known as black pepper, sansho, and windmill grass. The plant height is about 30-90 cm, dioecious, the male flowers are yellow, the female flowers are red-orange, 3-4 ovaries, and the fruit is oval. The ornamental value of pepper wood is high, mainly concentrated on the branches and leaves. The obovate opposite leaflets of about 0.6 cm are leathery, dark green and shiny, petite and exquisite, very cute. Green branches and leaves are suitable for pruning, landscaping, hedges or potted plants, and become a good material for flat and vertical greening in courtyards and parks. Pepperwood is generally propagated by cuttings, but the propagation by cuttings is affected by the material of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 李晓东徐浩张晓红
Owner SHENZHEN POLYTECHNIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com