Yeast engineering bacterium for expression recombination of rainbow trout antimicrobial peptide Oncorhyncin II and preparation method thereof

Abstract

The invention discloses Saccharomyces cerevisiae engineering bacteria for expressing recombinant rainbow trout antibiotic peptide Oncorhyncin II and the preparation method thereof. The preparation method comprises the steps as follows: introducing the coding genes of the mature peptide of the rainbow trout antibiotic peptide Oncorhyncin II into the Saccharomyces cerevisiae to obtain the strains capable of secretion-expressing the rainbow trout antibiotic peptide Oncorhyncin II. The Saccharomyces cerevisiae rainbow trout antibiotic peptide Oncorhyncin II is preserved in China General Microbiological Culture Collection Center on June 19th, 2008 with the preservation number of CGMCC No.2554. The Saccharomyces cerevisiae engineering bacteria can stably express the recombinant rainbow trout antibiotic peptide Oncorhyncin II, which has antibacterial activity to gram positive bacteria and gram negative bacteria, such as Escherichia coli, Staphylococcus aureusetc, and can be used for preparing feed additives, fresh-keeping agents, cosmetics and pharmaceutical products.

Description

technical field [0001] The invention relates to a yeast engineering bacterium in the technical field of microbial fermentation and a construction method thereof, in particular to a Saccharomyces cerevisiae engineering bacterium expressing recombinant rainbow trout antibacterial peptide Oncorhyncin II and a preparation method thereof. Background technique [0002] Unlike mammals, teleosts lack specialized lymphoid organs such as lymph nodes. Thus, compared with mammals, the secondary acquired immune response is weaker in teleosts, in other words fish are more dependent on the innate immune system, especially during ontogeny or in environments where antibodies cannot be produced. It is known that the factors involved in the non-specific immune response of fish mainly include some phagocytic cells such as macrophages and granulocytes, as well as protein molecules that mediate a series of immune or stress responses, such as complement, cytokines, and chemokines. and lysozyme an...

AI Technical Summary

Problems solved by technology

Although a large number of protein drugs have been successfully expressed in yeast expression systems, there have been no reports on the successful expression of recombinant rainbow trout antimicrobial peptide Oncorhyncin II using yeast expression systems, or yeast engineering bacteria that express recombinant rainbow trout antimicrobial peptide Oncorhyncin II and the report on the construction of said bacteria

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