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Potato test-tube plantlet assembly type fragment propagating method

A technology for test-tube seedlings and potatoes, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems affecting the large-scale popularization and application of virus-free potatoes, restricting the reproductive efficiency of test-tube seedlings, and cumbersome operations, etc., so as to reduce bacterial contamination. The probability of sterilization, the reduction of sterilization times, and the effect of improving efficiency

Inactive Publication Date: 2009-01-07
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome to operate, low in efficiency, and high in cost; it seriously restricts the propagation efficiency of test-tube seedlings, and affects the large-scale application of virus-free potatoes in potato production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1, potato green potato No. 9 virus-free test-tube seedling propagation

[0012] a. Basic seedling cultivation: potato test-tube seedlings are cultivated for 22-25 days at 20-22 ° C, 16 hours of light / 8 hours of dark cultivation, and each test-tube seedling grows to 5-6 leaves; about 30 seedlings per bottle;

[0013] b. Preparation of medium: prepare simplified MS medium, that is, MS medium containing only macronutrients and trace elements, add 6 g / L of agar powder, 30 g / L of sucrose, and adjust the pH value to 5.8; subpackage to culture Put it into the bottle, put it into an autoclave and sterilize it at 121°C for 20 minutes; take it out and place it flat, cool and solidify, and set aside;

[0014] c. Cluster cutting: On a sterile workbench, use sterilized tweezers to take out all the basic seedlings in the culture bottle at one time, and use sterilized scissors to cut off the basic seedlings at the same time according to their internode positions , cut into...

Embodiment 2

[0019] Embodiment 2, potato green potato No. 2 virus-free test-tube seedling propagation

[0020] a. Basic seedling cultivation: potato test-tube seedlings are cultivated for 22-25 days at 20-22 ° C, 16 hours of light / 8 hours of dark cultivation, and each test-tube seedling grows to 5-6 leaves; about 30 seedlings per bottle;

[0021] b. Preparation of medium: prepare simplified MS medium, that is, MS medium containing only macronutrients and trace elements, add 6 g / L of agar powder, 30 g / L of sucrose, and adjust the pH value to 5.8; subpackage to culture Put it into the bottle, put it into an autoclave and sterilize it at 121°C for 20 minutes; take it out and place it flat, cool and solidify, and set aside;

[0022] c. Cluster cutting: On a sterile workbench, use sterilized tweezers to take out all the basic seedlings in the culture bottle at one time, and use sterilized scissors to cut off the basic seedlings at the same time according to their internode positions , cut into...

Embodiment 3

[0027] Embodiment 3, the virus-free test-tube seedling propagation of chip type potato Atlantic variety

[0028] a. Basic seedling cultivation: potato test-tube seedlings are cultivated for 22-25 days at 20-22 ° C, 16 hours of light / 8 hours of dark cultivation, and each test-tube seedling grows to 5-6 leaves; about 30 seedlings per bottle;

[0029] b. Preparation of medium: prepare simplified MS medium, that is, MS medium containing only macronutrients and trace elements, add 6 g / L of agar powder, 30 g / L of sucrose, and adjust the pH value to 5.8; subpackage to culture Put it into the bottle, put it into an autoclave and sterilize it at 121°C for 20 minutes; take it out and place it flat, cool and solidify, and set aside;

[0030] c. Cluster cutting: On a sterile workbench, use sterilized tweezers to take out all the basic seedlings in the culture bottle at one time, and use sterilized scissors to cut off the basic seedlings at the same time according to their internode positi...

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Abstract

The invention relates to a cluster-type segment propagation method of potato test-tube plantlets. The method comprises the following steps: preparing a simplified MS culture medium, adding agar powder and sucrose in the amounts of 6 g / liter and 30 g / liter respectively, and adjusting the pH value to 5.8; packaging the resulting culture medium in a culture bottle respectively and sterilizing for 20 minutes in an autoclave at 121 DEG C; taking all the test-tube plantlets of another culture bottle, cutting off the plantlets at the same time by using a pair of sterilized scissors according to the internode positions of the plantlets, and transferring to the prepared culture bottle, wherein each bottle of plantlets is distributed in four new culture bottles and the stem segments in each culture bottle are 1.3 to 1.5 times of the plantlets; laying out the stem segments transferred to the new culture bottles; placing the sealed culture bottles in an incubator house for culture under such conditions that the temperature is in the range of 20 DEG C to 22 DEG C, the illumination period is 16 hours, the dark period is 8 hours and the culture period is 22 to 25 days; and repeating the previous steps until the desired yield is obtained.

Description

technical field [0001] The invention relates to a potato breeding method, in particular to a potato tube seedling breeding method. Background technique [0002] Potatoes are one of the most important food crops. The planting area of ​​potatoes in our province is more than 1 million mu per year, and it is the third largest planting crop. In the past, potato production has been affected by "potato degeneration" for a long time, and the excellent seed properties of new varieties have been continuously lost, resulting in long-term low potato yields and unguaranteed quality. With the popularization and application of potato detoxification technology, this problem has been gradually solved. However, the virus-free potatoes are easily infected with the virus again in field production, showing a "degeneration" phenomenon, and can no longer be used as seeds after three generations. This requires that the virus-free potato plantlets must be continuously provided in order to continuo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨永智王舰周云
Owner QINGHAI UNIVERSITY
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