Fed-batch feeding method in fermentation production of neomycin sulfate

Abstract

The invention discloses a fed-batch feeding method in a fermentation production of neomycin sulfate. The method comprises the following steps: step 1, sequentially arranging a flow meter and a pneumatic diaphragm valve on a feeding pipeline according to a feeding direction; step 2, determining a time interval H of each sampling; step 3, determining the total material supplementing amount S and calculating a material supplementing rate V; step 4, setting a material supplementing period t; step 5, determining a material supplementing instantaneous rate V; step 6, calculating starting t; step 7,calculating closing t; and step 8, completing fed-batch feeding in a fermentation production of neomycin sulfate. The provided fed-batch feeding method in a fermentation production of neomycin sulfateomits a metering tank and a large number of pipeline valves, accurate fed-batch feeding of a fermentation tank is achieved through the pneumatic diaphragm valve, a manual diaphragm valve, a blow-downvalve and an electromagnetic flowmeter, operation is easy, no sterilization dead angle exists, a risk of microorganism contamination of a feeding system is reduced, and sterilization times of a material supplementing system are reduced.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a fed-batch feeding method in the fermentative production of neomycin sulfate. Background technique [0002] At present, fed-batch feeding method (referring to a method of continuously adding some nutrients to the fermentation system during the batch fermentation process) is widely used in the antibiotic fermentation industry, but in the domestic fermentation production of neomycin sulfate The main reason is that in the neomycin sulfate fermentation process, a large amount of carbon source and nitrogen source with relatively high substrate concentration need to be supplemented as nutrients (such as sugar material), and the substrate concentration is as high as 40%, and there is a certain Viscous, if feed feeding is used, the feeding rate is low, and the sugar material is easy to block the pipeline valve, which brings inconvenience to maintenance and easily causes steri...

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned automatic metering cup feeding method reduces the labor intensity and the risk of human operation, it also avoids the problem of excessive one-time feeding to a certain extent. However, this set of feeding system involves a large number of pipeline valves, which is easy to form a dead angle for sterilization, and is more likely to cause the risk of bacterial contamination to the entire feeding system, and it is inconvenient to maintain. Substances with high concentration and viscosity, such as sugar, will often stick to the liquid level electrode, affecting the signal transmission of the liquid level electrode, causing problems such as inaccurate measurement or signal delay delays in feeding time

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