Method for preparing sodium gluconate through adopting semicontinuous fermentation technology

A technology of sodium gluconate and fermentation process, which is applied in the field of preparing sodium gluconate by using a semi-continuous fermentation process, which can solve the problems of no catalytic activity, low equipment utilization rate, and large steam consumption, so as to reduce the number of times of steam sterilization and reduce the The effect of steam consumption and increasing sodium acid content

Active Publication Date: 2013-10-16
黑龙江中瑯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the production of sodium gluconate by the catalytic oxidation method will produce by-products such as glucose dialdehyde and glucuronic acid, and after the catalyst used is recycled for a certain number of times, the catalytic efficiency will decrease, the conversion rate of glucose will be reduced, and the reaction time will be prolonged or even basically There is no catalytic activity, and the replacement of the catalyst will increase the production cost accordingly; while the traditional biological fermentation method to produce sodium gluconate also has the disadvantages of low equipment utilization, large steam consumption, and weak production continuity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Put the fermentation medium into the fermenter, insert the Aspergillus niger spore liquid (China Industrial Microorganism Strain Management and Preservation Center, CICC40417), and incubate:

[0022] The cultivation time is 15 hours, the tank pressure is controlled at 0.22MPa, the temperature is controlled at 35°C, the pH value is controlled at 6.8 within 0-12h, and 6.4 after 12h, and the ventilation ratio is controlled at 1.6 within 0-4h, 4-8h Control it within 1.8, and control it at 2.2 after 8 hours;

[0023] (2) Release 50% of the culture solution and supplement the same volume of fresh medium for cultivation:

[0024] The cultivation time is 6 hours, the tank pressure is controlled at 0.14MPa, the temperature is controlled at 33°C, the pH value is controlled at 6.5 within 0-4h, 6.0 after 4h, and the ventilation ratio is controlled at 1.0;

[0025] (3) step (2) is carried out in a cycle, and a total cycle is carried out 6 times;

[0026] (4) put the tank.

[...

Embodiment 2

[0030] (1) Put the fermentation medium into the fermenter, insert the Aspergillus niger spore liquid (China Industrial Microorganism Strain Management and Preservation Center, CICC40424), and incubate:

[0031] The cultivation time is 18 hours, the tank pressure is controlled at 0.18MPa, the temperature is controlled at 32°C, the pH value is controlled at 6.5 within 0-12h, and 6.0 after 12h, and the ventilation ratio is controlled at 1.5 within 0-4h, 4-8h Control it within 1.6, and control it at 1.8 after 8 hours;

[0032] (2) Release 20% of the culture solution and supplement the same volume of fresh medium for cultivation:

[0033] The cultivation time is 4.5 hours, the tank pressure is controlled at 0.16MPa, the temperature is controlled at 38°C, the pH value is controlled at 6.7 within 0-4h, 6.4 after 4h, and the ventilation ratio is controlled at 1.2;

[0034] (5) step (2) is carried out in a cycle, and a total cycle is carried out 9 times;

[0035] (6) put the tank.

...

Embodiment 3

[0039] (1) Put the fermentation medium into the fermenter, insert the Aspergillus niger spore liquid (China Industrial Microorganism Strain Management and Preservation Center, CICC40405), and incubate:

[0040] The cultivation time is 16 hours, the tank pressure is controlled at 0.20MPa, the temperature is controlled at 34°C, the pH value is controlled at 6.7 within 0-12h, and 6.2 after 12h, and the ventilation ratio is controlled at 1.5 within 0-4h, 4-8h Control it within 1.7, and control it at 2.0 after 8 hours;

[0041] (2) Release 30% of the culture solution and supplement the same volume of fresh medium for cultivation:

[0042] The cultivation time is 5.5 hours, the tank pressure is controlled at 0.15MPa, the temperature is controlled at 35°C, the pH value is controlled at 6.6 within 0-4h, 6.2 after 4h, and the ventilation ratio is controlled at 1.1;

[0043] (7) step (2) is carried out in a cycle, and a total cycle is carried out 7 times;

[0044] (8) put the tank.

...

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Abstract

The invention relates to a method for preparing sodium gluconate through adopting a semicontinuous fermentation technology. The method comprises the following steps: 1, putting a fermentation medium into a fermentation tank, accessing an Aspergillus niger spore liquid, and carrying out temperature maintenance culture; 2, discharging 20-50% of the obtained culture solution, and simultaneously supplementing the fresh medium which is isometric to the discharged culture solution to culture; 3, circulatingly carrying out step 2; and 4, discharging the obtained material from the tank. Compared with traditional fermentation technologies, the preparation method of the invention, which adopts the fermentation medium with the sugar concentration of 5-10% higher than sugar concentrations of traditional fermentation mediums, has the advantages of improvement of the content of sodium gluconate in the total fermentation liquid, reduction of the steam disinfection frequency of the fermentation tank and a pipeline thereof, steam consumption reduction, strong production continuity, guarantee of the uniformity and the continuity of post-extraction production, and finished product quality stability guarantee.

Description

technical field [0001] The invention relates to a method for preparing sodium gluconate, in particular to a method for preparing sodium gluconate using a semi-continuous fermentation process. Background technique [0002] Sodium gluconate, also known as sodium pentahydroxyhexanoate, is a white or light yellow crystalline powder, easily soluble in water, slightly soluble in alcohol, and insoluble in ether. Its molecular formula is C 6 h 11 o 7 Na, the relative molecular weight is 218.14. Sodium gluconate is a polyhydroxy organic acid salt with a wide range of uses. Due to its non-toxicity and wide source of raw materials, it has a wide range of uses in chemical, food, medicine, light industry and other industries, such as being used as an acid-base balance agent , water quality stabilizer, surface cleaning agent, cement participation agent, etc. [0003] At present, the preparation methods of sodium gluconate mainly include catalytic oxidation method and traditional biol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/58C12R1/685
Inventor 李悦明张希铭王道会薛元刚步长平徐建春
Owner 黑龙江中瑯生物科技有限公司
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