Color development liquid for peroxidase mensuration and preparation method thereof

A color-developing solution and color-developing technology, which can be used in biochemical equipment and methods, microbial determination/inspection, and analysis by chemically reacting materials, and can solve problems such as reduced practicability and limited detection sensitivity.

Inactive Publication Date: 2009-01-28
深圳生科原生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result is that various solvents greatly inhibit the reaction of TMB and peroxide, so that its detection sensitivity is limited in clinical

Method used

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  • Color development liquid for peroxidase mensuration and preparation method thereof
  • Color development liquid for peroxidase mensuration and preparation method thereof
  • Color development liquid for peroxidase mensuration and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0027] The method for preparing the chromogenic liquid system for the determination of peroxidase in the embodiment of the present invention comprises the following steps:

[0028] (1) Take carbamide peroxide, heavy metal ion complexing agent, and compounded 4-tert-butyl-4'-methoxydibenzoylmethane and add them into the buffer respectively and mix well as storage solution I for subsequent use;

[0029] Dissolve 3.3'5.5'-Tetramethylbenzidine in acetone, dissolve the resulting solution in ethanol, add the ethanol solution into the buffer and mix well, add sodium thiosulfate into the buffer, mix well as Storage solution II for standby;

[0030] (2) Mix storage solution I and storage solution II, adjust the pH value of the buffer solution, and then constant volume.

[0031] Both the storage solution I and the storage solution II are prepared from a buffer solution with a pH range of 2.5-5.5, which is only used as the above-mentioned basic dissolution buffer system, and has a great...

Embodiment 1

[0034] 1. Prepare storage solution I.

[0035] Take 500ml double distilled water. Add 14.72 grams of sodium citrate monohydrate crystals, shake and mix. Use 0.1mol / L hydrochloric acid to adjust the pH value of the solution to 5.0 to make a 0.05mol / L citric acid-sodium citrate buffer

[0036] Weigh 10 grams of EP-110, add it into the citric acid-sodium citrate buffer, shake and mix well.

[0037] Weigh Bassoon-17890.1 g, add it to citric acid-sodium citrate buffer, shake and mix.

[0038] Weigh 0.6 g of carbamide peroxide, add it into citric acid-sodium citrate buffer solution, shake and mix well, and use it as storage solution I for subsequent use.

[0039] 2. Prepare storage solution II.

[0040] Take 300ml of double distilled water, add 4.1g of sodium acetate trihydrate crystals, shake and mix. Use 0.1mol / L hydrochloric acid to adjust the pH value of the solution to 5.0. Make 0.05mol / L acetic acid-sodium acetate buffer solution.

[0041] Weigh 0.5 g of TMB and dissolve ...

Embodiment 2-13

[0046] The preparation process of the color development system with different component concentrations in Examples 2-13 is the same as that of Example 1, only the corresponding amount of the substance is added in the corresponding link of the preparation process. Refer to Table 1 for the final concentration of each component in the chromogenic liquid system solution of the example.

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PUM

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Abstract

The invention provides a visualization reagent architecture that is used for peroxydase measurement and a preparation method thereof. The visualization reagent architecture comprises urea peroxide, a heavy metal ion complexing agent and a compound of 4-tert-butyl-4'-methoxy-dibenzoylmethane, 3.3' 5.5'-tetramethylbenzidine and sodium hyposulfite, the preparation method of the architecture comprises the following steps: (1) a storage liquid I is prepared and ready for use, and a storage liquid II is prepared and ready for use; and (2) the storage liquid I and the storage liquid II are mixed, pH value of a buffering liquid is adjusted and volume is limited. Peroxides and chromophoric substrates in the visualization reagent architecture that is provided by the invention can coexist stably without reaction for a long time, thus leading the solution architecture to be easily stored and have consistent sensitivity during measurement.

Description

technical field [0001] The invention relates to a chromogenic liquid system for measuring peroxidase and a preparation method thereof. Background technique [0002] The peroxidase chromogenic solution system is a commonly used chromogenic tracer system in the research and detection fields of immunology and immunohistochemistry. At present, it is widely used in clinical and scientific research. The basic principle is: peroxide decomposes in the presence of peroxidase, and releases oxidized oxygen (O-), oxidized oxygen (O-) and the colorless hydrogen donor in the color system ( Chromogen) redox reaction occurs, the chromogen is oxidized, and the chromogen in the oxidized state produces color. After the reaction is completed, the color depth of the chromogenic system directly indicates the content of the detected substance in the reaction system. To achieve qualitative or quantitative detection. However, due to the high standard potential difference between the peroxide and...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/28
Inventor 张鹏唐晓玲张阳
Owner 深圳生科原生物有限公司
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