Uses of gamma-ray combined phTERT-HRP/IAA in medicament for treating cancer of larynx

A technology of γ-rays and drugs, which is applied in the field of medicine, can solve the problems of aggravating radiation damage to normal tissues around the tumor, damaging laryngeal function, and affecting the quality of life of patients, and achieves the effect of enhancing hTERT promoter activity and enhancing expression

CN101366950AInactive Publication Date: 2009-02-18WUHAN UNIV
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Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2009-02-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses application of the combination of gamma ray and phTERTp-HRP / IA in medicines for killing larynx cancer cells, and in particular relates to the enhancement of the gamma ray in promoter activity of hTERT gene and the synergetic effect of the combination of the gamma ray and the phTERTp-HRP / IA for killing the human larynx cancer cells. The invention studies levels of different radiation sensitivity cancer cells in cells and animals and detects the promoter activity and enzymatic activity, volume, weight and tumor inhibiting rate of transplanted tumor, tumor tissue HE coloration and apoptosis of lower-reach gene expression products to evaluate the synergetic killing effect when the gamma ray treatment and the hTERT promoter regulating-controlling HRP / IAA system gene treatment are jointly used. The result shows that when the gamma ray treatment and the phTERTp-HRP / IAA treatment are jointly used, the human larynx cancer cells are obviously killed.
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Description

technical field

[0001] The invention relates to the field of medical technology, more specifically to the application of gamma rays in enhancing the gene therapy drug mediated by the promoter of telomerase reverse transcriptase gene (hTERT), and gamma rays are used in enhancing the activity of hTERT promoter and enhancing the expression of downstream genes role. At the same time, it also involves the application of gamma rays and hTERT promoter to regulate the horseradish peroxidase (HRP) / indole acetic acid (IAA) system to produce a synergistic effect to destroy laryngeal cancer cells and inhibit the growth of laryngeal cancer cells. Background technique

[0002] Looking at the changing pattern of the disease spectrum at home and abroad, malignant tumors have become a major disease that seriously threatens human health. With the advancement of molecular biology, gene therapy has brought hope to defeat tumors. Various countries have carried out a lot of research on gene the...

Examples

Embodiment 1

[0032] Example 1 The effect of 2Gy γ-rays on enhancing the activity of hTERT promoter

[0033] 1 Experimental materials:

[0034] 1.1 Cells: Human laryngeal carcinoma cells (Hep-2, CCTCC number GDC009) were purchased from China Center for Type Culture Collection (CCTCC), and human laryngeal squamous cell carcinoma radiation-resistant strain (Hep-2R) was established by the applicant in previous work (See references: Luo Zhiguo, Zhou Fuxiang, Zhou Yunfeng, et al. The establishment of radiation-induced radiation-resistant squamous cell carcinoma cell lines and their biological characteristics. Chinese Journal of Radiation Oncology, 2005, 14: 208-212), conventional cell culture and passage .

[0035]1.2 Plasmid: The applicant has constructed the plasmid phTERTp-Luc in previous work (see references: Liao ZK, Zhou FX, Luo ZG, Zhang WJ, Xiong J, Bao J, Han G, ZhangMS, Xie CH, Zhou YF.Radio -activation of hTERT promoter in larynx squamous carcinoma cells: An'indirected-activator'str...

Embodiment 2

[0046] Example 2 The killing effect of γ-ray standard fractionated radiotherapy regimen combined with phTERTp-HRP / IAA on transplanted tumors of laryngeal carcinoma cells with different radiosensitivity

[0047] 1 Experimental materials:

[0048] 1.1 cells: same as Example 1

[0049] 1.2 Plasmid: The applicant has constructed plasmid phTERTp-HRP in previous work (see references: Liao ZK, Zhou FX, Luo ZG, Zhang WJ, Xiong J, Bao J, Han G, ZhangMS, Xie CH, Zhou YF.Radio -activation of hTERT promoter in larynx squamous carcinoma cells: An'indirected-activator'strategy in radio-gene-therapy.Oncol Rep.19(2008)281-286), internal reference plasmid pRL-TK and control plasmid pGL3-Basic were purchased from PROMEGA company .

[0050] 1.3 Experimental animals: BALB / C-nu nude mice aged 6 to 8 weeks were purchased from the Hubei Experimental Animal Center, each with a weight of about 16g, and were raised in an independent ventilation system in the Experimental Animal Center of Wuhan Univer...