Method for extracting and preparing total saponin of compound abnormal savda mature agent
A total saponin and abnormal technology, which is applied in the field of extraction and preparation of total saponin, can solve the problem of high total saponin content of abnormal black bile maturation agent, and achieve the effects of low cost, technological progress, and wide application range
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Embodiment 1
[0017] Take 3kg of dry powder of abnormal black gallbladder ripening agent, add 15000mL of 90°C hot water, stir for 30 minutes to fully dissolve, let stand at room temperature for 24 hours, and filter. Add 22,500 mL of 95% ethanol to the filtrate, stir for 30 minutes, let stand at 22°C for 24 hours, filter, concentrate the filtrate to a relative density of 1.30; Elute with 20% ethanol, discard the water and 20% ethanol eluate, then elute with 60% ethanol, collect the eluate; recover ethanol from the eluate under reduced pressure, concentrate until no alcohol smell; add 95% ethanol to the concentrate Dissolve, filter, decolorize and purify the filtrate with activated carbon, filter to obtain the total saponin extract of abnormal black gallbladder mature agent, concentrate under reduced pressure, vacuum dry, and pulverize to obtain the total saponin product of abnormal black gallbladder mature agent, colorimetric method The purity of total saponins was measured to be 58.2%.
Embodiment 2
[0019] Take 3 kg of dry powder of abnormal black gallbladder ripening agent, add 16000 mL of 86°C hot water, stir for 35 minutes to fully dissolve, let stand at room temperature for 32 hours, and filter. Add 22000mL 90% ethanol to the filtrate, stir for 35 minutes, let stand at 20°C for 32 hours, filter, concentrate the filtrate to a relative density of 1.28; , 30% ethanol for elution, discard water and 30% ethanol eluate, then elute with 65% ethanol, collect eluate; eluate is decompressed to recover ethanol, concentrated to no alcohol smell; concentrate is added with 95% Dissolved in ethanol, filtered, and the filtrate was decolorized and purified by activated carbon, filtered to obtain the total saponin extract of the abnormal black gallbladder mature agent, concentrated under reduced pressure, spray-dried, and pulverized to obtain the total saponin product of the abnormal black gallbladder mature agent, colorimetric The purity of total saponins measured by the method was 67...
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