Cell specimen screening and separation device

Abstract

A cell specimen screening and separation device belongs to the technical field of cell analysis appliances, solves the problem of major-diameter cells in an extracted specimen, and is provided with a split main body (1); left and right circular / conical seal heads (2 and 3) which are formed through wholistic processing and provided with extrusion pipes are arranged at two ends of the split main body (1), and are provided with detachable left and right seal covers (6 and 7) matched with the left and the right circular / conical seal heads; filter screen embedding mechanisms (11) embedded with thick and thin filter screens (8 and 9) are respectively arranged on inner walls at two end parts of the main body (1); the cell specimen screening and separation device is non-electric, is convenient to operate, and can ensure that large and small particles in the cell specimen are separated out rapidly and stably, the successful extraction of large-aperture cells is realized, and the whole operation process is sealed, so that the health of operators is facilitated greatly; and the cell specimen screening and separation device is a disposable product, is low in cost, and is easy to manufacture, store, transport and apply on a large scale, and raw materials are easy to access.

Description

technical field [0001] The invention belongs to the technical field of instruments for cell analysis, and relates to a device for screening and separating cell samples. Background technique [0002] At present, more than 10 million pieces of cell specimens (mixed solution) with large and small particles are separated in China every year. There are many cell interference substances in the original specimens, including large and small particles, which need to be removed by certain physical means. Therefore, The quality of the sample cell particle separation directly affects the accuracy of the experimental results. There are two methods for extracting large-diameter cells in specimens, both of which are imported from abroad. The first method uses microporous membrane filtration suction equipment to directly suck the mixed liquid. Small particles and a certain liquid are under negative pressure. Through the filter membrane (the filter pore size is less than 10um, the strength ...

AI Technical Summary

Problems solved by technology

There are two methods for extracting large-diameter cells in specimens, both of which are imported from abroad. The first method uses microporous membrane filtration suction equipment to directly suck the mixed liquid. Small particles and a certain liquid are under negative pressure. Through the filter membrane (the filter pore size is less than 10um, the strength of the membrane is not enough after the pore size is increased), and the large and medium particles are retained together with the large-diameter cells, so as to realize the separation of large-diameter cells and small particles. After that, the large-diameter cells are separated. Particles are treated separately; the second method is to directly separate the mixed liquid with a centrifuge, so as to realize the separation of large-diameter cells from large and small particles; the disadvantage of the first method is that because the microporous The filter pore size of the membrane is small. If it is enlarged, the strength of the membrane is not enough and it is easy to break. Therefore, the membrane pores of the microporous membrane are often blocked due to too many small particles in the mixed liquid, which may easily cause incomplete separation or separation failure. And large particles need to be treated separately. The disadvantage of the second method is that the operation is too cumbersome, and it is necessary to take another test tube, first mark the number, then add the sample liquid, then add the separation liquid, and pour off the upper liquid after centrifugation. Keep the pellets at the bottom of the test tube, add the separation liquid for the second time, and pour off the upper part of the liquid after centrifugation with a greater centrifugal force

Although it has been centrifuged twice, it is difficult to remove large particles with a specific gravity similar to that of large cells. In addition, operator violations are prone to occur, such as numbering mistakes, which increase the possibility of errors and cannot be well separated. Large particles are separated, and thus affect the final experimental results, leading to wrong conclusions

At the same time, the above two separation methods both have to open the cover (especially the second method), and the unknown infectious source that may exist in the cell specimen is easy to infect the operator.

The cost of importing from abroad (including supporting devices) is high, and some people imitate it, which brings a lot of trouble and even leads to lawsuits

Images