Italian penicillium mitochondria extraction method

An extraction method of Penicillium italicum, which is applied in the field of mitochondrial extraction of Penicillium italicum, can solve the problems of agricultural production economic loss, fruit rot, etc., and achieve the effect of high yield and good activity

Inactive Publication Date: 2019-02-12
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Penicillium italicum causes a large number of fruit rots during the postharvest storage and transportation of citrus, which brings huge economic losses to agricultural production

Method used

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  • Italian penicillium mitochondria extraction method
  • Italian penicillium mitochondria extraction method
  • Italian penicillium mitochondria extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention will be described in detail below by way of examples.

[0028] 1. Experimental materials

[0029] 1.1 Strains

[0030] Penicillium italica

[0031] 1.2 Materials and reagents

[0032] Sucrose, Mannitol, Pyruvate, Malate, Succinate, Hepes, Tris, MOPS, EGTA, DTT, No Fattyacid BSA, Percoll, ADP.

[0033] 2. Optimization of the extraction buffer system of Penicillium italicum mitochondria

[0034] 2.1 Preparation of Penicillium spore suspension and culture of mycelia

[0035] Activate the Penicillium italicum standard strain on the potato dextrose agar medium, take the activated Penicillium strain with an inoculation needle and add sterile water to prepare a concentration of 1.0×10 6 Penicillium spore suspension in cfu / mL. Take 5ml Penicillium spore suspension and inoculate it into 250ml potato dextrose liquid medium, culture on a shaking table at 26°C and 120r / min for 24h.

[0036] 2.2 Optimization method for the components of Penicillium itali...

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Abstract

The invention discloses an Italian penicillium mitochondria extraction method. A used extraction buffering solution is Hepes-KOH buffering solution with the pH of 7.4 and comprises 0.6M mannitol, 0.2percent BSA, 2mM EGTA and 2mM DTT. By utilizing an established Italian penicillium mitochondria extraction buffering system, the Italian penicillium mitochondria can be successfully extracted, the mitochondria are high in yield and good in activity, so that further research for the electron transfer chains, proteomics and genetic mechanism of the Italian penicillium mitochondria is possible, and the foundation is set for further researching the antibacterial mechanism of a sterilizing agent adopting the mitochondria as a target point for Italian penicillium.

Description

technical field [0001] The invention relates to a method for extracting mitochondria of penicillium italicum. Background technique [0002] Citrus fruits are very popular in the daily sales of fruits for their unique aroma and taste and play an important role in nutrition supply and health promotion. Penicillium italicum causes a large number of fruit rots during postharvest storage and transportation of citrus, which brings huge economic losses to agricultural production. Through the extraction of Penicillium italicum mitochondria, the function of Penicillium italicum mitochondria can be explored, so as to further explore the antibacterial mechanism of fungicides targeting mitochondria on Penicillium italicum, which can more effectively control the disease. [0003] Usually, the mitochondrial extraction method is to first break the cells to homogenize them, and then separate and purify them by differential centrifugation and density gradient centrifugation. The extraction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/80
CPCC12N1/14
Inventor 王熠彭丽桃
Owner HUAZHONG AGRI UNIV
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