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Plant polysaccharide and preparation method and use thereof

A technology of plant polysaccharides and polysaccharides, applied in plant raw materials, plant/algae/fungus/moss components, drug combinations, etc., can solve the problems of limited clinical application and large side effects

Inactive Publication Date: 2009-03-25
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the anticoagulant effect of heparin, a relatively high concentration is required in the body to exert its drug effect, and the side effects are large, which limits its clinical application.

Method used

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  • Plant polysaccharide and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of polysaccharide PS-1

[0028] Houttuynia cordata, wild chrysanthemum, capillary, peilan, and grass fruit are combined according to the ratio of 15:6:15:10:3, totaling 9.6Kg. Extract by cold immersion in 95% ethanol, filter, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and defreeze the supernatant with trichloroacetic acid The protein is centrifuged, the supernatant is dialyzed with water for 3 days, the dialysate is concentrated to a small volume, and then ethanol is added to reach an alcohol content of 80%, centrifuged, precipitated and freeze-dried to obtain crude polysaccharide. The crude polysaccharide was dissolved by adding an appropriate amount of distilled water, centrifuged, and the supernatant was washed with a DEAE-cellulose column (Cl -1 type, 30×2.5cm) chromatography for preliminary separation. Elute with distilled wat...

Embodiment 2

[0030] Example 2 Classical pathway complement inhibition test

[0031] Take the serum of healthy adult male volunteers and use VBS 2+ Buffer (barbital buffer, pH=7.4, containing 0.5mM Mg 2+ and 0.15mM Ca 2+ ) was diluted 1:10 as a source of complement for the classical pathway. Rabbit anti-sheep erythrocyte antibody in VBS 2+ The buffer was diluted to 1:1000 as hemolysin; sheep red blood cells (SRBC) stored in Alsever solution were configured as 2% SRBC. Precisely weigh about 1mg of PS-1, add VBS 2+ The buffer was dissolved and diluted to 8 concentrations. 100 μL of PS-1 solution with different concentrations and 100 μL of complement at 1:10 were pre-incubated at 37°C for 10 minutes, and then 200 μL of LVBS was added in sequence 2+ Buffer, 100 μL hemolysin (1:1000) and 100 μL 2% SRBC were placed in a low-temperature high-speed centrifuge after being placed in a 37°C water bath for 30 minutes, and centrifuged at 5000 rpm and 4°C for 10 minutes. Take 200 μL of the superna...

Embodiment 3

[0032] Example 3 Alternative Pathway Complement Inhibition Test

[0033] Serum was taken from healthy adult male volunteers and mixed with VBS-Mg-EGTA buffer (barbital buffer, pH=7.4, containing 5mM Mg 2+ and 8mM EGTA) diluted 1:10, as a source of complement for the alternative pathway. Rabbit red blood cells stored in 3.8% sodium citrate solution were prepared into 2% rabbit red blood cells with VBS-Mg-EGTA buffer solution. Accurately weigh about 1 mg of PS-1, add VBS-Mg-EGTA buffer, and dilute to 8 concentrations. 150 μL of different concentrations of PS-1 solution and 150 μL of 1:10 complement were pre-incubated at 37°C for 10 minutes, then 200 μL of 2% rabbit red blood cells were added, and placed in a low-temperature high-speed centrifuge after 30 minutes in a water bath at 37°C. Centrifuge for 10 min. Take 200 μL of the supernatant from each tube in a 96-well plate, and measure the absorbance at 405 nm. At the same time, a PS-1 control group (150 μL PS-1 solution of ...

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Abstract

The invention belongs to the Chinese medicine field, in particular to a plant polysaccharide PS-1, the preparation method thereof and the application in the preparation of anti-complement medicine. The invention obtains PS-1 through separating and extracting from the water extract of Chinese herbal compound used for preventing severe acute respiratory syndrome (SARS) and composed of houttuynia cordata, chrysanthemum indicum L, artemisia capillaries, eupatorium fortune and Amomum tsao-ko in the proportion of 15:6:15:10:3; as proven in the experiment that PS-1 respectively has inhibition effect on the classical pathway and alternative pathway of the activation of complement, acts on the ingredients of C1q, C1r, C1s, C2, C3,C4,C5 and C9, and has no anticoagulative effect. The plant polysaccharide PS-1 can be further used as an active ingredient for the preparation of new anti-complement medicine.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and relates to plant polysaccharides, in particular to a plant polysaccharide and its preparation method and application in the preparation of anti-complement drugs. Background technique [0002] Under normal physiological conditions, the function of complement is mainly to attack foreign pathogens, remove immune complexes, and maintain the balance of the body. However, the abnormal activation of the complement system will cause the excessive response of the human immune system, resulting in the damage and inflammation of the body's own normal tissues. Studies have shown that diseases associated with excessive activation of complement include rheumatoid arthritis, senile dementia, systemic lupus erythematosus (SLE), rejection after organ transplantation, multiple organ failure syndrome, acute respiratory distress syndrome (ARDS )wait. According to reports, in severe atypical pneumonia...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K36/9064A61P37/06A61P31/14A61P11/00
Inventor 陈道峰张婷章蕴毅
Owner FUDAN UNIV
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