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Composition containing peptide as active ingredient

一种有效成分、组合物的技术，应用在含有肽作为有效成分的组合物领域，能够解决糖尿病恶化、糖耐量异常等问题，达到促进糖原储存、增进运动能力、提高持久力的效果

Active Publication Date: 2009-05-06
MEIJI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, if the blood sugar level suddenly rises after a meal and persists (postprandial hyperglycemia) all year round, it may cause abnormal glucose tolerance and worsen diabetes

Method used

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  • Composition containing peptide as active ingredient
  • Composition containing peptide as active ingredient
  • Composition containing peptide as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0050] Dissolve 50 g each of casein, soybean protein, wheat gluten, whey protein and beef in 1 L of water. After adjusting the pH value of each solution to 7.0, it was heated to 50° C. and kept warm. 500 mg of protease derived from Bacillus (Protease M "Amano", Amano Enzymes, Inc.) and 500 mg of protease derived from Aspergillus (Protease N "Amano", Amano Enzymes, Inc.) were added to the solution and incubated for 8 hours, Then heat for 10 minutes to inactivate the protease.

[0051]The obtained solution was made into a powder by freeze-drying. Dilute the powder 1000 times (volume ratio) in 0.1% trifluoroacetic acid (TFA) aqueous solution, and use LC / MS under the following conditions to analyze Ile-Leu, Ile-Trp, Ala-Leu, Val-Leu, Gly-Leu, The contents of Asp-Leu, Lys-Ile, Leu-Leu, Ile-Ile, Leu-Ile, Ile-Asn, Leu-Ala, Leu-Glu, Leu-Val and Ile-Val were quantified.

[0052] Table 1 shows the production amount (mg) of each peptide per 1 g of protein. Peptides as shown in Table ...

manufacture example 2

[0070] Dissolve 50g of whey protein in 1L of water. 1) 500 mg of protease derived from Bacillus (Protease M "Amano", Amano Enzymes, Inc.), 2) 500 mg of protease derived from Aspergillus (Protease N "Amano", Amano Enzymes, Inc.) .), 3) 500mg trypsin (Novo company), 4) 500g pepsin (Wako Pure Chemical Industries, Ltd.), 5) 500mg compound flavor protease (Flavorzyme) (Novo company), 6) 500mg Aspergillus source protease (umamizaime ( (trade name), Amano Enzymes, Inc.), 7) 500 mg of Aspergillus-derived protease (Protease A "Amano", AmanoEnzymes, Inc.), and 8) 500 mg of Aspergillus-derived protease (Protease P "Amano", Amano Enzymes, Inc.), for hydrolysis of whey protein (Table 2). After heat-inactivating the protease, the resulting solution was freeze-dried to make a powder. The powder was diluted 1000 times (volume ratio) in 0.1% trifluoroacetic acid (TFA) aqueous solution, and Ile-Leu, Ile-Trp, Ala-Leu, Val- Leu, Gly-Leu, Asp-Leu, Lys-Ile, Leu-Leu, Ile-Ile, Leu-Ile, Ile-Asn, Le...

manufacture example 3

[0076] Dissolve 50g of whey protein in 1L of water. After adjusting the pH value of the solution to 7.0, it was heated to 50° C. and kept warm. 500 mg of protease derived from Bacillus (Protease M "Amano", Amano Enzymes, Inc.) and 500 mg of protease derived from Aspergillus (Protease N "Amano", Amano Enzymes, Inc.) were added to the solution, incubated for 8 hours, and then Heat for 10 minutes to inactivate the protease. Then, 1 L of 0%, 50%, 60%, 70%, 80%, 90%, 95% ethanol (ethanol relative to ethanol aqueous solution total Volume %), Ile-Leu and Ile-Trp were extracted. The extract was concentrated with a concentrating evaporator, and then freeze-dried to make a powder. The powder was diluted 1000-fold (volume ratio) in 0.1% trifluoroacetic acid aqueous solution, and Ile-Leu and Ile-Trp were quantified using LC / MS under the above conditions.

[0077] The results are shown in Table 3. The extract extracted with 90% ethanol contained the most Ile-Leu and Ile-Trp.

[0078]...

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PUM

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Abstract

A composition which contains a peptide having an effect of promoting sugar intake as the active ingredient and is used for promoting sugar intake; and a composition which contains a dipeptide having leucine and / or isoleucine as the active ingredient. These compositions are effective in preventing or treating diabetes or an increase in blood sugar level, promoting glycogen storage, improving physical strength, improving athletic ability, enhancing stamina or relieving fatigue.

Description

technical field [0001] The present invention relates to a composition containing a peptide as an active ingredient. Furthermore, the present invention also relates to a food or pharmaceutical composition containing the aforementioned composition. Moreover, this invention also relates to the manufacturing method of the said composition. Background technique [0002] Diabetes is a general term for a group of metabolic diseases characterized by insufficient insulin action and persistent hyperglycemia. Diabetes mellitus is caused by a certain combination of decreased insulin sensitivity and decreased insulin secretion, and characteristic abnormalities of sugar, lipid, and protein metabolism occur. Recently, diabetes has been recognized as a lifestyle-related disease, and improvement can be expected through appropriate countermeasures. However, if diabetes develops to a severe degree, complications such as neurological disorders, retinopathy, or kidney disease may occur, and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A23L1/305A61P3/10
CPCA23V2002/00A61K38/05A23L1/3051A61K38/018A61K38/011A23L33/175A61P3/02A61P3/08A61P3/10A61P43/00A23V2200/316A23V2250/0626A23V2250/0628A61K38/00
Inventor 森藤雅史仓重惠子三本木千秋
Owner MEIJI CO LTD
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