Method for measuring minimum inhibiting concentration of heavy metal to microorganism

A technology that inhibits concentration and microorganisms, applied in the field of environmental microorganisms, can solve problems such as the inability to fully reflect the ability of bacteria to tolerate heavy metals, and achieve reliable inhibitory effects

Inactive Publication Date: 2009-05-27
HUNAN UNIV
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  • Abstract
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Problems solved by technology

This assay does not adequately represent the ability of bacteria to tolerate heavy metals,

Method used

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  • Method for measuring minimum inhibiting concentration of heavy metal to microorganism

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Embodiment 1

[0013] Prepare liquid medium suitable for microbial growth with gradient concentration of heavy metal ions, and insert the strains in the logarithmic growth phase into the sterilized above-mentioned medium under sterile conditions, and place them at 28°C, 150r / min cultured in an incubator. Take out the bacterial solution at an interval of 6-8 hours, and measure the OD under the ultraviolet spectrophotometer 600 ; Determination of OD multiple times 600 The concentration at which there is no increase in value is the minimum inhibitory concentration.

[0014] Utilize this measurement method to measure the MIC of ten kinds of bacteria (isolated from the endophytic bacteria of plant Solanum nigrum) preserved in the laboratory, the results are as follows:

[0015]

[0016] +: good resistance; +—: resistant; —: no resistance.

[0017] The minimum inhibitory concentrations of ten kinds of endophytic bacteria in plant Solanum nigrum were determined in chromium, cadmium and zinc i...

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Abstract

The invention provides a method for quickly measuring the minimum inhibitory concentration of heavy metals on microbe, comprising: respectively preparing liquid culture medium adding with heavy metal ion gradient concentration which is suitable for growth of the microbe, moving strain in logarithmic phase to sterilized culture medium for culture in sterile conditions, measuring OD value by using UV spectrophotometry, and obtaining the minimum inhibitory concentration which has no increase change in OD values measured several times. The method is favorable to quickly select strains, and is more reliable for measuring the inhibitory of heavy metals on microbe.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and relates to a method for rapid determination of the minimum inhibitory concentration of heavy metals on microorganisms. Background technique [0002] As a key step in the screening of bacteria with tolerance and removal effect on heavy metals, the determination of the minimum inhibitory concentration (MIC) requires rapidity and high precision. There are no patents or inventions dedicated to the determination of the minimum inhibitory concentration. The closest one is 01103281.2 on the rapid determination of microbial susceptibility, which mainly judges the susceptibility of microorganisms to the test substance according to the changes of microbial cell number and aggregation form. This method of determining the minimum inhibitory concentration of microorganisms to heavy metals requires a long time, generally two days to one week, because the microorganisms need to be inoculated on ...

Claims

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Application Information

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IPC IPC(8): G01N21/17C12Q1/02
Inventor 罗胜联万勇肖潇郭函君陈亮曹喆苏峰
Owner HUNAN UNIV
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