Animal model construction method of bile pigment calculus guinea pig

A technology of animal model and construction method, which is applied in the fields of medical science, surgery, diagnosis, etc., can solve the problems of low modeling success rate, high mortality rate, and long modeling time, so as to reduce modeling time and improve modeling success The effect of high rate and modeling efficiency and good application prospect

Inactive Publication Date: 2009-06-17
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above two methods have certain problems. One is that the modeling time is long (6 weeks) and the mortality rate is too high (nearly 60%); , the modeling success rate is relatively low

Method used

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  • Animal model construction method of bile pigment calculus guinea pig
  • Animal model construction method of bile pigment calculus guinea pig
  • Animal model construction method of bile pigment calculus guinea pig

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Materials and methods

[0018] 1.1 Drugs and reagents

[0019] Lincomycin hydrochloride (SPECTINOMYCIND lincomycin hydrochloride), injection, produced by Shanghai Hefeng Pharmaceutical Co., Ltd.; guinea pig feed and guinea pig stone-causing feed, provided by Shanghai Slack Experimental Animal Co., Ltd., each serving of stone-causing feed contains: normal feed 45.43 g and 4.57 g of lithogenic drugs (the lithogenic drugs contain: 1 g of casein, 1.5 g of sucrose, 1 g of lard, 1 g of microcrystalline cellulose, 0.2 g of cholic acid, and 0.05 g of cholesterol).

[0020] 1.2 Experimental animals

[0021] Common-grade male guinea pigs (guinea-pig) were purchased from Yingen Rabbit Breeding Room, Tairi Town, Fengxian County, Shanghai, weighing 450±50g.

[0022] 2. Construction of animal models

[0023] Twenty-four male guinea pigs were randomly divided into normal control group, pigment gallstone model group 1, and pigment gallstone model group 2, with 8 animals in each g...

Embodiment 2

[0035] 1. Materials and methods

[0036] 1.1 Drugs and reagents

[0037] Lincomycin hydrochloride (SPECTINOMYCIND lincomycin hydrochloride), injection, produced by Shanghai Hefeng Pharmaceutical Co., Ltd.; guinea pig feed and guinea pig stone-causing feed, provided by Shanghai Slack Experimental Animal Co., Ltd., each serving of stone-causing feed contains: normal feed 44.43 g and 5.57 g of lithogenic drugs (the lithogenic drugs contain: 1 g of casein, 1.5 g of sucrose, 1 g of lard, 2 g of microcrystalline cellulose, 0.2 g of cholic acid, and 0.05 g of cholesterol).

[0038] 1.2 Experimental animals

[0039] Common-grade male guinea pigs (guinea-pig) were purchased from Yingen Rabbit Breeding Room, Tairi Town, Fengxian County, Shanghai, weighing 340±30g.

[0040] 2. Construction of animal models

[0041] Twenty-four male guinea pigs were randomly divided into normal control group, pigment gallstone model group 3, and pigment gallstone model group 4, with 8 animals in each g...

Embodiment 3

[0051] 1. Materials and methods

[0052] 1.1 Drugs and reagents

[0053] Lincomycin hydrochloride (SPECTINOMYCIND lincomycin hydrochloride), injection, produced by Shanghai Hefeng Pharmaceutical Co., Ltd.; guinea pig feed and guinea pig stone-causing feed, provided by Shanghai Slack Experimental Animal Co., Ltd., each serving of stone-causing feed contains: normal feed 44.43 g and 5.57 g of lithogenic drugs (the lithogenic drugs contain: 2 g of casein, 1.5 g of sucrose, 1 g of lard, 1 g of microcrystalline cellulose, 0.2 g of cholic acid, and 0.05 g of cholesterol).

[0054] 1.2 Experimental animals

[0055] Common-grade male guinea pigs (guinea-pig) were purchased from Yingen Rabbit House, Tairi Town, Fengxian County, Shanghai, weighing 300±20g.

[0056] 2. Construction of animal models

[0057] Twenty-four male guinea pigs were randomly divided into normal control group, pigment gallstone model group 5, and pigment gallstone model group 6, with 8 animals in each group.

[...

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Abstract

The present invention relates to a method for constructing animal model, and especially discloses a method for constructing a cavy animal model with bile pigment stone. According to the invention, a cavy with body weight of 300-600g is selected. Lincomycin hydrochloride is injected to the skin of thigh root of cavy with a dosage of 6-600mg / kg / day. The cavy is fed by stone-forming feed stuff in the model constructing period. The invention uses the stone-forming feed stuff for replacing common feed stuff, reduces the model constructing time and increases the success rate of model constructing. The method for constructing animal model can enlarge the application prospect of bile pigment stone model.

Description

technical field [0001] The invention relates to a construction method of an animal model, in particular to a construction method of a guinea pig animal model of pigment gallstone. technical background [0002] Cholelithiasis is a common disease that seriously endangers human health. Cholelithiasis can occur in the gallbladder, intrahepatic bile duct and common bile duct. Clinical gallstones can be roughly divided into cholesterol stones and pigment stones. [0003] Nowadays, people generally establish animal models of cholelithiasis and use scientific methods to find the cause of cholelithiasis by studying model animals, prevent gallstone formation, the onset of symptoms carried by gallstones, and relapse after gallstone removal, and fundamentally find a thorough and effective way to prevent and treat cholelithiasis. Method. [0004] The modeling method of guinea pig gallstone model caused by lithogenic feed is: guinea pigs weighing 250-300g are fed with lithogenic feed fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61B90/00
Inventor 张静喆刘建文顾宏刚章学林梁晓强马恩伟李炯林天碧孙逊蒋红伟方邦江朱培庭山冬梅房一时叶依依李长龙吴宏忠邓婉利孙丽娟徐文于有军王芳王劲李艾斯
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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