Cardiac thrombosis animal model and construction method thereof

Abstract

The invention relates to the technical field of medicine, in particular to a cardiac thrombosis animal model and a construction method thereof. The construction method comprises the following steps: introducing a chlorophosphonate preparation into the abdominal cavity of an animal; after 0.5-2 days, carrying out ligation on the anterior descending branch of the left coronary artery of the animal in the anesthetic state; and after ligation for 0.5-2 days, introducing the chlorophosphonate preparation into the abdominal cavity of the animal again. The mouse myocardial infarction model is prepared by a method of myocardial infarction modeling, injection of a small amount of chlorophosphonate lipidosome before and after an operation and removal of macrophages in a short period, and the success rate of left ventricular thrombus formation of a modeling mouse reaches 90% or above. The method is simple and easy to implement and can be widely popularized and applied to experimental animals of various body types, the left ventricular thrombosis forming rate is high, the effect is stable, the modeling time is short, and the cardiac thrombosis animal model is closer to the pathophysiological state of left ventricular thrombosis after myocardial infarction clinically.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an animal model of cardiac thrombosis and a construction method thereof. Background technique [0002] With the intensification of population aging, cardiovascular diseases, especially acute myocardial infarction, have become one of the leading causes of death in my country, showing an increasing trend year by year. Although percutaneous coronary intervention technology is becoming more and more popular, most patients with myocardial infarction can However, some patients still have myocardial ischemia and necrosis due to excessive ischemia time, which in turn causes ventricular systolic dysfunction, leading to serious complications such as left ventricular thrombosis, and left ventricular thrombus formed at any time There is a risk of obstructing the outflow tract or falling off into the systemic circulation, causing cerebral infarction or ischemic necrosis of other important orga...

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Problems solved by technology

[0003] At present, there are few methods for making animal models of left ventricular thrombus, and the process is cumbersome

A New Zealand scholar reported the preparation method of the left ventricular thrombus model: firstly, the thrombus was formed by drawing rabbit blood and placing it outside the body for 3 hours, then cut the sternum midline and sewed a purse on the surface of the heart, and then injected the thrombus formed in vitro through the purse. Left ventricle, this method is complicated to operate, not only needs a lot of time to make thrombus, but also requires ultrasound guidance during the operation, and the thrombus cannot attach to the side wall of the left ventricle. Significantly different left ventricular thrombus after infarction

In addition, some domestic scholars have reported a similar modeling method: one end of the thrombus is injected into the heart cavity, and the other end is left outside the heart and fixed. This method can make the model animals survive for a long time, but it also makes the thrombus too stable and difficult to fall off. , far removed from the clinical pathophysiological state

Some foreign scholars forced the blood flow to stop for a few minutes by inducing cardiac arrest in pigs, blood stasis formed left ventricular thrombus, followed by cardiopulmonary resuscitation and ventricular defibrillation to restore hemodynamics. This method makes important organs of the body, especially the brain Ischemia time is too long, causing irreversible damage, and the formed thrombus is easy to dissolve, and the effect is unstable

[0004] In summary, the current methods used to prepare animal models of left ventricular thrombus are not ideal, and there is no method suitable for the construction of a smaller mouse model of left ventricular thrombus. Therefore, a new simple modeling method is urgently needed for follow-up scientific research

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