Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing asiatic centella total aglycone by asiatic centella total glycosides acid hydrolysis

A technology of oxalic acid and centella asiatica, which is applied in the direction of steroids and organic chemistry, can solve the problems of time-consuming and labor-intensive consumption, and consume a large amount of solvents, so as to protect cardiovascular and cerebrovascular, induce tumor cell apoptosis, and facilitate industrialization Effect

Inactive Publication Date: 2009-06-17
ZHEJIANG UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, the preparation method of total aglycones of Centella asiatica is mainly obtained by direct extraction from Centella asiatica grass, supplemented by purification processes such as extraction and chromatography, which not only consumes a lot of solvents, but also is time-consuming and laborious.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing asiatic centella total aglycone by asiatic centella total glycosides acid hydrolysis
  • Method for preparing asiatic centella total aglycone by asiatic centella total glycosides acid hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Add 500mL of a mixed solution of methanol, water and sulfuric acid to a 1L three-necked flask with a stirring and water condenser, wherein the volume percentage of methanol is 10%, and the equivalent concentration of sulfuric acid is 0.1N; the three-necked flask is placed in a constant temperature water bath at 65°C , start stirring and condensing water, add 10g of asiaticoside raw material, hydrolyze for 24h; hydrolyzate is concentrated under reduced pressure to remove methanol, precipitate and filter to obtain a precipitate, which weighs 3.7g after drying; collect and dry Add 18.5mL of methanol to dissolve the precipitate, decolorize with activated carbon, filter, add 333mL of water to the filtrate to crystallize, and obtain crystals after filtration, and then vacuum dry the crystals to obtain 2.5g total aglycone products of Centella asiatica. The mass content of the total aglycon in Centella is 84.1%.

Embodiment 2

[0030]Add 500mL of a mixed solution of methanol, water and hydrochloric acid to a 1L three-necked flask with a stirring and water condenser, wherein the volume percentage of methanol is 20%, and the equivalent concentration of hydrochloric acid is 0.5N; the three-necked flask is placed in a constant temperature water bath at 65°C , stir and condense water, add 20g of asiaticoside raw material, hydrolyze for 10h; hydrolyzate is concentrated under reduced pressure to remove methanol, precipitate and filter to obtain precipitate, which weighs 7.4g after drying; collect and dry Add 74mL of ethanol to the precipitate to dissolve, decolorize with activated carbon, filter, add 1184mL of water to the filtrate to crystallize, obtain crystals after filtration, and then vacuum dry the crystals to obtain 5.2g total aglycone products of Centella asiatica, the product is analyzed by HPLC The mass content of total grass aglycones is 90.1%.

Embodiment 3

[0032] Add 500mL of a mixed solution of methanol, water and sulfuric acid to a 1L three-necked flask with a stirring and water condenser, wherein the volume percentage of methanol is 30%, and the equivalent concentration of sulfuric acid is 1.0N; the three-necked flask is placed in a constant temperature water bath at 60°C , stir and condense water, add 30g of asiaticosides raw material, hydrolyze for 8h; hydrolyzate is concentrated under reduced pressure to remove methanol, precipitate and filter to obtain precipitate, which weighs 11.2g after drying; collect and dry Add 168mL of ethanol to the precipitate to dissolve, decolorize with activated carbon, filter, add 2352mL of water to the filtrate to crystallize, obtain crystals after filtration, and then vacuum dry the crystals to obtain 7.8g total aglycone products of Centella asiatica, the product is analyzed by HPLC The mass content of total grass aglycon is 90.3%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing asiaticoside by acid hydrolysis of centella selected triterpenes, which comprises the following steps: 1) adding centella selected triterpenes raw material into a mixed solution of an organic solvent I, water and inorganic acid for hydrolysis at a temperature of between 45 and 80 DEG C for 1 to 24 hours; 2) concentrating the hydrolysate solution at reduced pressure to remove the organic solvent I, depositing and filtering the hydrolysate solution to obtain deposit; and 3) drying the deposit, adding the deposit into an organic solvent II for dissolution, decoloring the solution by active carbon, filtering the solution, adding water into filtrate for crystallization, filtering the filtrate to obtain crystals, and drying the crystals in vacuum to obtain the asiaticoside product. The asiaticoside product has high yield and purity, and can be used as bulk drug or further separated into different asiatic acid products; the asiaticoside product taken as a medicine can be used to treat skin wound, resist depressive illness and Alzheimer's disease, protect liver and veins of heart and brain, induce tumour cell apoptosis and so on; meanwhile, the method has simple technological process, small investment, and easy industrialization.

Description

technical field [0001] The invention relates to a method for preparing total aglycones of centella asiatica by hydrolyzing total glycosides of centella asiatica. Background technique [0002] Centella asiatica (L.) Urban is a plant of the genus Centella asiatica in the Umbelliferae family. It has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine has been It has a history of two thousand years. Centella asiatica is bitter in the mouth, pungent, cold in nature, returns liver, spleen, kidney, stomach meridian. According to ancient pharmacopoeia records, it is mainly used to treat diseases such as damp-heat jaundice, heatstroke diarrhea, sand and blood, carbuncle sores, and bruises. Modern pharmacological research and application have shown that Centella asiatica can be used to treat depression, skin wounds, gastric ulcers, infectious hepatitis, sk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J63/00
Inventor 吕秀阳郑兴芳李音
Owner ZHEJIANG UNIV