An online activation device for biological bacteria and its application method

Abstract

An online activation device for biological bacteria and its use method, in which a temperature zone of 0°C to 4°C and a temperature zone of 20°C to 25°C are set in the device, and two sets of freeze-dried powder are set in the temperature zone of 0°C to 4°C for storage and quantification Control unit and nutrient solution storage; two sets of freeze-dried powder storage and quantitative control units are respectively connected to corresponding luminescent bacteria activation reactors through freeze-dried powder tubes; two luminescent bacteria activation reactors, Activated luminescent bacteria storage tanks, buffer storage tanks. The invention realizes the on-line activation of the biological bacteria, thereby solving the problems of grasping the optimal life cycle of the biological bacteria and prolonging the continuous detection as much as possible. The accuracy of the detection result is improved. It can provide the on-line activation of photobacteria that is suitable for online automatic monitoring in harsh environments. It is suitable for resource protection, environmental monitoring, biology, environmental protection, petrochemical industry, soil, microorganisms and other occasions that require online activation of fungi.

Description

technical field [0001] The invention belongs to an online activation device for biological bacteria and a use method thereof. Background technique [0002] In the fields of resource protection, environmental monitoring, biology, environmental protection, petrochemical industry, soil, microorganisms and other fields, it is necessary to use online activated fungi, but the activation of bacteria is usually realized in the laboratory by two methods: slant planting and liquid mixing and stirring. The existing problem is that the activation time and conditions are controlled manually, which is not suitable for the occasion of continuous use of biological bacteria in a system. If there is no online activation device, the biological bacteria required for the continuous operation system are activated in advance and then injected into the system. Because the lifespan of bacteria is limited and the longest lifespan is known, the continuous operation of the system does not require human...

AI Technical Summary

Problems solved by technology

[0002] In the fields of resource protection, environmental monitoring, biology, environmental protection, petrochemical industry, soil, microorganisms, etc., it is necessary to use online activation of bacteria, but usually the activation of bacteria is achieved in the laboratory by planting on an inclined plane and by mixing and stirring liquids. The problem is that the activation time and conditions are controlled manually, which is not suitable for the continuous use of biological bacteria in a system

[0004] However, the above-mentioned test instruments made of luminescent bacteria in the past all have the problem of not being able to activate the bacteria online, and there are some problems as follows: For example, the special test instruments produced by Beckman and the luminescent bacteria test are matched, but they are used in the laboratory and the price is high. And the requirements for the environment are too strict

[0005] 1. Since the CWDZF-I online analyzer does not have an online activation device, the luminescent bacteria required for each test are activated in advance and then injected into the instrument, because the lifespan of the bacteria is limited, and the longest lifespan is 117 Hours, so the longest time for continuous testing without human intervention is 5 days. According to the detection results of the life curve of luminescent bacteria, the best luminescence time of luminescent bacteria is the period from 20 hours to 36 hours after activation. CWDZF-I type analysis The instrument does not address the issue of optimal life cycle and continuous detection as long as possible

[0006] 2. The pipeline of CWDZF-I online analyzer uses medical catheters and medical containers, and uses the principle of hanging salt water to realize the injection of luminescent bacteria. There will be a problem that other bacteria will appear inside the pipeline and compete with luminescent bacteria for resources.

affecting the accuracy of the test results

[0007] 3. The CWDZF-I online analyzer originally only had one temperature zone, which could not ensure that the activity of the luminescent bacteria was in the best state, so the luminous intensity of the luminescent bacteria during the test was insufficient, which affected the quality of the test

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