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Uses of MAGEA4 gene

A gene and gene chip technology, applied in the application field of MAGEA4 gene, can solve the problem of unclear relationship between liver cancer and achieve accurate diagnosis.

Inactive Publication Date: 2009-07-29
CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The relationship between the MAGEA4 gene and the occurrence of liver cancer is still unclear, and further research is needed

Method used

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  • Uses of MAGEA4 gene
  • Uses of MAGEA4 gene
  • Uses of MAGEA4 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] RT-PCR assay was used to detect the expression of MAGEA4 gene in liver cancer tissues.

[0035] 1. Tissue isolation

[0036] The tissues used in the experiment came from 72 surgical patients with HBV-positive primary liver cancer expressing alpha-fetoprotein (RT-PCR confirmed that AFP was positive in liver cancer and negative in adjacent tumors). Once the surgically resected liver was isolated from the body, the lesion and surrounding paracancerous tissues 5 cm away were quickly excised and stored in liquid nitrogen (-80°C). The diagnosis of cancer and paracancer is based on pathological diagnosis.

[0037] 2. Total RNA extraction kit

[0038] The RNA extraction reagent uses TRIzol reagent (GIBCO / BRL), which is produced based on the one-step extraction method with acidic phenol. The utensils and water used for RNA extraction must be RNase-free to ensure an RNase-free environment in the experiment.

[0039] 3. RNA extraction steps

[0040] Dry-bake the pestle, homog...

Embodiment 2

[0051] Embodiment 2 real-time quantitative PCR experiment

[0052] Relative quantitative method was used to detect the expression difference of MAGEA4 gene in liver cancer samples (Thermal CyclerDiceTM Real Time System TP800, Takara; Premix Ex Taq™, Takara). Real-time quantitative PCR detection showed that the expression level of MAGEA4 gene in liver cancer tissues was significantly higher than that in paracancerous tissues, and the t test showed P<0.01. The results of this experiment show that real-time quantitative PCR can be used to diagnose liver cancer: design PCR primers for the MAGEA4 gene to detect the RNA content of the MAGEA4 gene in tumor tissues. A high RNA content indicates a high possibility of liver cancer, and vice versa.

Embodiment 3

[0053] Example 3 in situ hybridization

[0054] Use the MAGEA4 gene probe to carry out in situ hybridization with the tumor section, the stronger the positive hybridization signal, the higher the possibility of suffering from liver cancer. The experimental steps are as follows: the liver tumor tissue is taken, embedded in OCT, quick-frozen in liquid nitrogen, and frozen in a constant temperature refrigerator for sectioning, and the frozen section is further used for in situ hybridization.

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Abstract

The invention discloses an application of MAGEA4 gene which is used for preparing products for diagnosing liver cancer and medicines for treating the cancer. The MAGEA4 gene of the invention can be used as the peculiar marker gene for diagnosing liver cancer, facilitates the diagnosis on the liver cancer to be more accurate and rapid and provides new therapeutic targets and effective medicines for preventing and treating liver cancer.

Description

technical field [0001] The present invention relates to a gene, in particular to the application of a MAGEA4 gene. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage. Once symptoms appear, it is often in the middle and late stages. Therefore, the treatment is difficult and the curative effect is poor. Generally, the survival time after the onset is only 6 months. It is called "the king of cancer". Therefore, improving the level of early diagnosis of liver cancer, choosing scientific and reasonable treatment methods, and taking effective measures to prevent recurrence and metastasis will be the three key links to change this situation. [0003] At present, the most important tumor marker for the diagnosis of primar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/577A61K31/713A61K48/00A61K38/17A61K39/00A61P35/00
Inventor 韩泽广黄健邓庆
Owner CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
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