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Special culture medium of inducing human embryo stem cell

A technology of human embryonic stem cells and differentiation medium, which is applied in the field of special medium for inducing human embryonic stem cells to differentiate into liver cells in vitro, which can solve the problems of short survival period, shortage of donors, and limited treatment methods

Active Publication Date: 2012-10-03
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of such diseases mainly depends on orthotopic liver transplantation. However, a series of problems such as the extreme shortage of donors, death related to transplantation and surgery itself, life-long administration of immunosuppressants and the fatal complications caused by them are extremely serious. The earth limits the widespread development of this treatment
Hepatocyte transplantation and bioartificial liver as an auxiliary method of liver transplantation can partially alleviate the above problems, but the source cells also face the short survival period and are difficult to expand in large quantities, especially with the prolongation of culture time, the liver function gradually decreases Therefore, it is imminent to seek a suitable source of hepatocytes

Method used

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  • Special culture medium of inducing human embryo stem cell
  • Special culture medium of inducing human embryo stem cell
  • Special culture medium of inducing human embryo stem cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Directed differentiation of human embryonic stem cells into hepatocytes in vitro

[0029] The following media were used in the examples:

[0030] Differentiation medium I (embryoid body medium): on the basis of DF-12 medium (Gibco), 20% (15-25% is acceptable, volume percentage concentration, V / V) serum substitute (SR, Gibco), 0.1 mM (0.05-0.15 mM is acceptable) β-mercaptoethanol (Gibco) and 1 mM (0.5-1.5 mM is acceptable) glutamine (Gibco), pH 7.2-7.6. To increase nutrition, 0.5-1.5% (volume percent concentration, V / V) of non-essential amino acids (HyClone) can be added to the differentiation medium I.

[0031] Differentiation medium II (definitive endoderm differentiation medium): on the basis of DF-12 medium, 1% (0.5-1.5% is acceptable, volume percentage concentration, V / V) fetal bovine serum (FBS) is added, 0.1mM (0.05-0.15mM is acceptable) β-mercaptoethanol, 1mM (0.5-1.5mM is acceptable) glutamine and 100ng / mL activin A (activin A), pH7.2-7.6.

[0032]...

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Abstract

The invention discloses a special culture medium for differentiating human embryonic stem cells into hepatic cells by in vitro inducement, comprising a differentiating culture medium I, a differentiating culture medium II, a differentiating culture medium III, and a differentiating culture medium IV. The invention has the advantages of simple operation, low cost, high differentiating efficiency, and the like, exerts important action in medical fields, and has wide application prospects.

Description

technical field [0001] The invention relates to a special culture medium for inducing differentiation of human embryonic stem cells, in particular to a special culture medium for inducing human embryonic stem cells to differentiate into liver cells in vitro. Background technique [0002] End-stage liver disease caused by various reasons (viruses, drugs, tumors and genetic diseases, etc.) seriously threatens human health. According to statistics, more than 110,000 patients die of end-stage liver failure each year nationwide. At present, the treatment of such diseases mainly depends on orthotopic liver transplantation. However, a series of problems such as the extreme shortage of donors, death related to transplantation and surgery itself, life-long administration of immunosuppressants and the fatal complications caused by them are extremely serious. The earth limits the widespread development of this treatment. Hepatocyte transplantation and bioartificial liver as an auxili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08C12N5/071
Inventor 裴雪涛裴海云王韫芳白宗良杨印祥习佳飞岳文南雪白慈贤
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY