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Method for keeping activity of probiotic

A probiotics, vitality technology, applied in the direction of fixed on/in organic carrier, animal feed, animal feed, etc., can solve the problems of probiotics loss of vitality, loss of efficacy, inability to use heat-sensitive microbial products, etc., to reduce Activity, improve utilization, improve the effect of ecological environment

Inactive Publication Date: 2011-08-24
孙瑞椒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after microbial products are heated, most of the probiotics lose their vitality and lose their efficacy
This makes it impossible to use heat-sensitive microbial products in the processing and granulation of aquatic microencapsulated bait

Method used

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  • Method for keeping activity of probiotic
  • Method for keeping activity of probiotic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 (taking lactobacillus as example)

[0014] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (preparation method with prior art), the reaction time is 12 hours, the reaction temperature is 55 ° C, and the reaction pH is 6.0. Under this condition, better Microporous starch (pore size is about 1 μm. The ratio of the cavity volume of microporous starch to the total volume of starch is greater than 50%), and 100 g of this microporous starch is used to adsorb (also call embedding) 50 g of lactic acid bacteria in its cavity Inside.

[0015] Then use the above-mentioned substances plus the total weight of 100kg of nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) as the core material, and use 1kg of chitosan dissolved in 400ml of lactic acid and 40kg of aqueous solution as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying ...

Embodiment 2

[0016] Embodiment 2 (taking lactobacillus as example)

[0017] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (using prior art), the reaction time is 12H, the reaction temperature is 55°C, and the reaction pH is 6.0. Under this condition, better microporous starch can be prepared (The microporous size is about 1 μm. The ratio of the cavity volume of the microporous starch to the total starch volume is greater than 50%). Use this microporous starch 100g to adsorb (also claim to embed) 55g lactobacillus in its cavity.

[0018] Then use the above-mentioned substances plus the total weight of 100kg of nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) as the core material, and use 1kg of chitosan dissolved in 400ml of lactic acid and 40kg of aqueous solution as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying method. The temperature of t...

Embodiment 3

[0019] Embodiment 3 (taking lactobacillus as example)

[0020] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (using prior art), the reaction time is 12H, the reaction temperature is 55°C, and the reaction pH is 6.0. Under this condition, better microporous starch can be prepared (The microporous size is about 1 μm. The ratio of the cavity volume of the microporous starch to the total starch volume is greater than 50%). Use this microporous starch 100g to adsorb (also claim to embed) 60g of lactobacilli in its cavity.

[0021] Then use the above-mentioned substances plus the total weight of 100kg of nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) as the core material, and use 1kg of chitosan dissolved in 400ml of lactic acid and 40kg of aqueous solution as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying method. The temperature of...

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Abstract

The invention relates to a method for keeping activity of probiotic. The probiotic is adsorbed into holes of microporous starch to generate a microporous adsorbate, and the microporous adsorbate and a mixture of nutrients required by aquatic seedlings form a core material; chitosan is used as a wall material, wherein the chitosan is insoluble in water but soluble in 1 percent lactic acid solution; and a microcapsule is prepared by using a spraying and drying method, the grain diameter of the microcapsule is less than 150mu m, and the ratio of the wall material to the core material is (3-10):100. The method can prevent the inactivation of the probiotic, and can play a role in slow release. The aperture of the micropore is 1mu m; the microporous starch, the ratio of the micropore cavity volume of which to the total volume of the starch is more than 50 percent, is used for adsorbing the probiotic into the holes of the microporous starch; the adsorbate of the microporous starch, which adsorbs the probiotic, is used as the core material; and the chitosan is used as a coating of the wall material. The method has the advantages that the microcapsule prepared by micropore adsorption and envelope can effectively keep the activity of the envelope during processing and manufacturing through experiment so as to develop the application prospect of the probiotic in aquatic feed.

Description

Technical field: [0001] The invention relates to a method for maintaining the vitality of probiotics. The invention belongs to the technical field of feed and feed additives. Background technique: [0002] Microbial preparations are also called live bacterial preparations (Biogen), probiotics, and probiotics (Probitics). Under the guidance of microecological theory, they are composed of beneficial microorganisms (PM) such as Lactobacillus, Bacillus, and yeast. After compound culture, Biological preparations or live bacterial preparations produced by special processes such as fermentation, drying, processing, etc., which can supplement the normal microorganisms that are lacking or lacking in the intestinal tract of animals in terms of quantity or type, adjust or maintain the micro-ecological balance in the intestinal tract, improve and enhance the body’s Immunity function, improve the body's anti-stress ability. The mechanism of action of microbial products is mainly manife...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04A23K10/18
Inventor 孙瑞椒袁艾李锁丽
Owner 孙瑞椒
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