Beauty composition containing vaccinium vitis-idaea extract and application thereof
A red bean lingonberry and extract technology, which is applied in the direction of drug combination, medical preparations containing active ingredients, applications, etc., can solve the problems of difficult extraction of active ingredients, complex product ingredients, limited application effects, etc., and achieve non-sensitization , improve skin health, and treat hyperpigmentation
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Embodiment 1
[0072] The preparation of embodiment 1 red bean lingonberry extract I
[0073] Soak 1000g red bean lingonberry with 3 times the amount of water, add 0.1% cellulase, stir, place in a constant temperature water bath at 40°C for 2 hours, and filter to obtain the extract. The extract was subjected to ultrafiltration membrane filtration (molecular weight 2000). The concentrated solution after membrane filtration is purified by HP20 macroporous adsorption resin, the product is eluted with 60% ethanol, concentrated and then spray-dried to obtain anthocyanin fraction.
[0074] Using the slag obtained under the above extraction conditions as a raw material, heat extraction with 75% ethanol to obtain a yellow paste. The paste was subjected to silica gel column chromatography to collect the resveratrol fraction.
[0075] The above anthocyanin part and resveratrol part were combined to obtain red bean lingonberry extract I, which was composed of 15% anthocyanin, 60% proanthocyanidin and...
Embodiment 2
[0076] The preparation of embodiment 2 red bean lingonberry extract II
[0077] 1000g red bean lingonberry fruit is used as a raw material, and 2% ethanol hydrochloric acid aqueous solution is used as a solvent for extraction at 40° C., and the extract is directly applied to a resin column. The resin adopts XAD16 macroporous adsorption resin. Wash the product with 90% ethanol, collect the eluate, concentrate in vacuo to remove the ethanol, and then spray dry to obtain the anthocyanin part.
[0078] Using the slag obtained under the above extraction conditions as a raw material, heat extraction with absolute ethanol to obtain a yellow paste. The paste was subjected to silica gel column chromatography to collect components rich in resveratrol, and recrystallized with 95% ethanol to obtain the resveratrol fraction.
[0079] Combining the above anthocyanin part and resveratrol part, the red bean lingonberry extract II is obtained, and its composition is: 5% anthocyanin, 10% proa...
Embodiment 3
[0080] The preparation of embodiment 3 red bean lingonberry extract III
[0081] 1000g red bean lingonberry fruit is used as a raw material, and 60% ethanol aqueous solution is used as a solvent for extraction at 40° C., and the ethanol is recovered from the extract and put on a resin column. Resin adopts CHPA20 ion exchange resin. Wash the product with 90% ethanol, collect the eluent, concentrate in vacuo to remove the ethanol, and then spray dry to obtain the anthocyanin part.
[0082] Using the slag obtained under the above extraction conditions as a raw material, heat extraction with 95% ethanol to obtain a yellow paste. The paste was subjected to silica gel column chromatography to collect the resveratrol fraction.
[0083] The above anthocyanin part and resveratrol part are combined to obtain red bean lingonberry extract III, which consists of 10% anthocyanin, 35% proanthocyanidin and 10% resveratrol.
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