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Field rapid detection test strip for tobacco compound virus and preparation method thereof

A technology for detecting test strips and composite viruses, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of unavailable rapid detection in the field and single function, and achieve the effect of comprehensive functions, convenient use, and high reliability

Inactive Publication Date: 2009-10-14
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are only suitable for laboratory testing and cannot be used for rapid field testing
And the current detection test strips of tobacco virus, each test strip can only detect one kind of tobacco virus, and the function is single

Method used

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  • Field rapid detection test strip for tobacco compound virus and preparation method thereof
  • Field rapid detection test strip for tobacco compound virus and preparation method thereof
  • Field rapid detection test strip for tobacco compound virus and preparation method thereof

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Experimental program
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Embodiment Construction

[0033] Embodiment list is as table 1:

[0034] Table 1 is a list of embodiments of the present invention

[0035] Table 1

[0036]

[0037]

[0038]

[0039]

[0040]

[0041]

[0042] The steps to use the test strip: take 0.1-0.3g tobacco leaf sample, the leaf area is about 3-5cm 2 , After grinding in a plastic bag with a buffer, insert the water-leading end of the test strip into the test sample, and the sample crawls to the other end of the test paper. If only the quality control line appears red on the test strip and the detection line does not develop color, the result is negative; if both the quality control line and the detection line appear red on the test strip, the result is positive; If the test lines are not colored, it means that the test strip has failed and the result is invalid.

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Abstract

The invention discloses a field rapid detection strip for tobacco complex virus. Colloidal gold tomography detection test paper is fixed on an appropriate position of a PVC plate; a water diversion material is connected with the lower end of the colloidal gold tomography detection test paper to form a water absorption paper layer; the water absorption material is connected with the upper end of the colloidal gold tomography detection test paper; a colloidal gold antibody combo glass fiber film is arranged below the water diversion material to be spliced with the colloidal gold tomography detection test paper; the assembled PVC plate is longitudinally cut into test strips being 5-7mm in width by a cutting machine, and the test strips are installed in a plate casing, i.e. a sample pad. The invention further discloses a preparation method of the test strip. The invention can detect a sample with the weight of 0.1-0 .3g under an optimum temperature of 15-35 DEG C, and the sample is equivalent to about 3-5cm<2> of the area of a leaf; tobacco leaves are grinded in a plastic bag filled with buffer solution, then the test strips are immersed for 30 minutes and then the results are obtained; and the false positive rate is less than 1 percent. The invention has convenient use, is especially suitable for field rapid detection, and has higher reliability.

Description

technical field [0001] The utility invention relates to the field of tobacco virus detection, in particular to a test strip for rapid detection of tobacco complex virus, and also relates to a preparation method of the test strip. Background technique [0002] Since the 1990s, tobacco virus disease has occurred frequently in my country's smoking areas, causing serious economic losses, and has become the most threatening disaster in tobacco production. In 2003, the economic loss of my country's tobacco due to tobacco virus disease was as high as 504.19459 million yuan. At present, tobacco virus disease also presents the new characteristics of increasing types of viral diseases and co-infection of multiple viruses. Common co-infection viruses include tobacco mosaic (TMV), cucumber mosaic (CMV), and potato virus Y (PVY). Since there is no effective prevention and control measures for tobacco virus disease so far, the prevention and treatment of tobacco virus disease should focu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/532G01N33/558
Inventor 邱立友李富欣王豹祥刘欣高玉千戚元成
Owner HENAN AGRICULTURAL UNIVERSITY
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