Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof

A technology of soil-borne fungus and solid particles, which is applied in botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc. It can solve the problems that the quality of solid culture is not easy to control and hinder the large-scale production of Trichoderma inoculum. , to achieve the effect of high recurrence rate, high success rate and strong operability

Active Publication Date: 2009-10-21
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the production of liquid-solid two-phase inoculum, the quality of solid culture is not easy to control, which hinders the large-scale production of Trichoderma inoculum

Method used

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  • Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof
  • Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof
  • Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] ——Laboratory test

[0062] In the process flow chart, from the slant strain to the shake flask bacterium slurry is the preparation stage of the laboratory liquid bacterium slurry, and after the bacterium slurry is connected to the solid, it is a workshop-style production process. details as follows:

[0063] 1. Cultivation of plate strains

[0064] According to the aseptic operation, pick the hyphae that have produced a large number of spores from the activated plate strains, place them on the plate at 3 to 5 points, and culture them under light at 27.5±1°C for 5 to 7 days. In the process of culturing after inoculation, pay attention to check the plates regularly every day. If there is contamination or abnormal growth cultures, they should be eliminated in time. In order to avoid the emergence of cultures with inconsistent spore production due to uneven illumination, the number of stacked layers can be reduced, such as 2 to 3 layers of petri dishes, and one layer can ...

Embodiment 2

[0080] ——Workshop Pilot Test

[0081] The pilot test process in the workshop is improved on the basis of the small laboratory test. The process steps are as follows:

[0082] 1. The culture of the plate strains, 2. The preparation of the liquid strains are the same as in Example 1.

[0083] ③ Liquid fermentation culture

[0084] Put the liquid strain into the fermenter containing the liquid fermentation medium SDIS at a volume ratio of 2% to 5%, at 28±1°C, ventilate with the medium:air volume ratio of 1:1.6~2.2, and rotate at 120 Stir at rpm, and cultivate for 36 to 42 hours.

[0085] ④ Solid fermentation culture

[0086] Inoculation: Inoculate the qualified fermentation broth in the solid fermentation medium RS at a mass ratio of 20% to 30%. When inoculating, use a mixer sterilized by steam, and stir while adding fermented liquid, until the material is grasped and squeezed vigorously until water drips out from the crevices of the fingers (water content 50% to 60%).

[00...

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Abstract

The invention discloses trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and a preparation method thereof, and aims to provide an effective, nontoxic, safe and residue-free bio-control microbial inoculum which is simple and convenient to use. The strain for producing the microbial inoculum is trichoderma harzianum TR13 with a preserving number of CGMCC No.2849. The preparation method comprises: (1) rejuvenescence of the strain; (2) preparation of the strain; (3) fermentation culture of a liquid; (4) fermentation culture of a solid; and (5) preparation.

Description

technical field [0001] The invention relates to biological pesticides, more specifically, the invention relates to a biological agent for preventing and treating tobacco soil-borne fungal diseases and a preparation method thereof. Background technique [0002] Tobacco soil-borne diseases (soil borne tobacco diseases) are a large class of tobacco diseases that are more harmful. The pathogens of these diseases can survive and reproduce in the tobacco planting soil, and infect roots, root shins, or pass through roots, Root shin infection causes root, root shin lesions, or whole plant lesions, and its occurrence is characterized by years of prevalence and aggravation year by year. Among these soil-borne diseases, fungal soil-borne diseases occur more frequently and are more harmful, and some have become important economic diseases in tobacco agricultural production. Such fungal diseases include tobacco black shank (Phytophthora parasitica var. nicotianae), damping-off (Pythium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/12A01P3/00C12N1/14C12R1/885
Inventor 方敦煌王革方力马永凯孔光辉
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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