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Regeneration type artificial blood vessel based on in-situ self stem cell technology and preparation method thereof

A technology of artificial blood vessels and stem cells, applied in the field of biomedicine, can solve the problems of no nano bionic materials and no technical reports.

Active Publication Date: 2009-11-25
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0024] However, bioprinting technology is still in the stage of basic research. What kind of material is the bio-paper sheet, what is the specific composition of the cell or / and cytokine bio-ink, and how to achieve the stacking are all theoretical. Discussions and assumptions, no specific nano-bionic materials that can be successfully prepared by bioprinting directly using cells have yet appeared, and no related technical reports have been seen.

Method used

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  • Regeneration type artificial blood vessel based on in-situ self stem cell technology and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Preparation of regenerative artificial blood vessels based on in situ autologous stem cell technology:

[0089] (1) Preparation of electrospinning solution, hydrosol solution containing specific antibody and / or cytokine and / or drug:

[0090] Select L-polylactic acid and ε-polycaprolactone as the electrospinning solution, the ratio of the two is 50:50, as a copolymerized polymer material, dissolve in hexafluoroisopropanol, and the mass percentage concentration is 3%; the crosslinking agent solution is selected as 0.1 M Calcium chloride solution; the hydrosol solution containing cytokines adopts endothelial growth factor alginate solution and angiogenin alginate solution. The mass percent concentration of the endothelial growth factor in the endothelial growth factor alginate solution is 100 ppm.

[0091] (2) Electrospinning is used to prepare the inner layer nano-bionic stent of the artificial blood vessel, and the hydrosol solution containing specific antibodies and / or...

Embodiment 2

[0110] Preparation of regenerative artificial blood vessels based on in situ autologous stem cell technology:

[0111] (1) Preparation of electrospinning solution, hydrosol solution containing specific antibody and / or cytokine and / or drug:

[0112] Select silk fibroin and gelatin as the electrospinning solution, the ratio of the two is 50:50, as a copolymerized polymer material, dissolve in formic acid, and the concentration is 10%; the cross-linking agent solution is selected from 100 mg / ml water-soluble carbodiimide solution; The hydrosol solution of the factor adopts the collagen and polyanion solution of the cytokine, wherein the collagen concentration is 1%, and the polyanion adopts polyglutamic acid with a concentration of 50 mg / ml. The mass percent concentration of the CD34 antibody in the hydrosol solution is 10 ppm.

[0113] (2) Electrospinning is used to prepare the inner layer nano-bionic stent of the artificial blood vessel, and the hydrosol solution containing sp...

Embodiment 3

[0131] Preparation of regenerative artificial blood vessels based on in situ autologous stem cell technology:

[0132] (1) Preparation of electrospinning solution, hydrosol solution containing specific antibody and / or cytokine and / or drug:

[0133] Select L-polylactic acid and ε-polycaprolactone as the electrospinning solution, the ratio of the two is 50:50, as a copolymerized polymer material, dissolved in hexafluoroisopropanol, the concentration is 3%; the crosslinking agent solution is selected from 0.1M chlorine Calcium solution; the hydrosol solution containing cytokines adopts endothelial growth factor alginate solution and angiogenin alginate solution. The mass percent concentration of the endothelial growth factor in the endothelial growth factor alginate solution is 100 ppm.

[0134] (2) Electrospinning is used to prepare the inner layer nano-bionic stent of the artificial blood vessel, and the hydrosol solution containing specific antibodies and / or cytokines and / or ...

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Abstract

The present invention discloses a regeneration type artificial blood vessel based on an in-situ self stem cell technology and a preparation method thereof. The artificial blood vessel is a structure composed of at least three connected bionic support layers, wherein the bionic support layer is prepared by nanometer bionic supports and hydrosol attached to the nanometer bionic supports; the structure is a concentric circle loop and has an inner layer, an insulating layer and an outer layer outwards; the hydrosol of the inner layer and the outer layer are packaged with one or more specificity antibodies and / or cytokines and / or adhered short peptides and / or medicaments. The preparation method includes preparing the inner layer by an electrostatic spinning; preparing the insulating layer on an external surface of the inner layer; preparing the outer layer on an external surface of the insulating layer. The artificial blood vessel of the invention may be degraded safely and automatically during a wound surface repair process, and defect blood vessel is repaired completely during the degradation process, thus a regenerative process and a normal differentiation process are identical, and a good repairing effect will be obtained.

Description

technical field [0001] The invention relates to a regenerative artificial blood vessel based on in situ autologous stem cell technology and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Vascular lesions, especially occlusive lesions of coronary arteries, are one of the main causes of death in humans. The main treatment method is vascular bypass surgery, which requires vascular grafts of various diameters as repair materials. According to the performance of natural blood vessels and considerations as a vascular substitute, the American Society of Vascular Surgery listed the following conditions for artificial blood vessels. The safety conditions include: stable physical properties in the living body; chemically stable and non-toxic; no foreign body reaction; no cancer induction; no allergic reaction; high fatigue strength, simple manufacture and low cost; simple disinfection. In addition to the conditions for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06A61L27/40A61L27/52A61L27/54A61L27/56A61L27/58
Inventor 徐弢袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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