Method for cleaning and reusing microdissection sample-collecting tubes

A technology for microdissection and collection tubes, applied in cleaning methods and utensils, cleaning methods using liquids, chemical instruments and methods, etc., can solve the problems of inability to remove small molecular substances, damage the adhesion of collection tubes, etc., and save scientific research The effect of saving money, saving the number of collection tubes, and lowering the price

Active Publication Date: 2011-10-05
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional mechanical washing, high temperature and high pressure washing, strong acid and strong alkali washing, etc. will damage the adhesion of the collection tube. Ultraviolet light and cobalt 60 irradiation can only inactivate microorganisms, but cannot remove small molecular substances.

Method used

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  • Method for cleaning and reusing microdissection sample-collecting tubes
  • Method for cleaning and reusing microdissection sample-collecting tubes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method of cleaning and reusing the microdissection specimen collection tube can be implemented in sequence as follows:

[0030] (1) Add xylene accounting for 1 / 2 of the volume of the collection tube into the collection tube, cover the tube cap, vortex for 3 minutes, open the tube cap, and discard the xylene;

[0031] (2) Add suspension A (mixed by 1.5% hydrochloric acid, 30% alcohol, 0.25mol / L EDTA in a volume ratio of 1:97:1) accounting for 2 / 3 of the volume of the collection tube into the collection tube, and add 50 mg of 0.1mm silica particles). Cover the tube cap, vortex for 2.5 minutes, open the tube cap, and empty the collection tube;

[0032] (3) Add double-distilled water accounting for 1 / 2 of the volume of the collection tube into the collection tube, add 50 mg of silica particles with a diameter less than 0.1 mm at the same time, cover the tube cap, vortex for 2.5 minutes, open the tube cap, pour Empty collection tube;

[0033] (4) Repeat step (2) and s...

Embodiment 2

[0039] The method of cleaning and reusing the microdissection specimen collection tube can be implemented in sequence as follows:

[0040] (1) Add xylene accounting for 2 / 3 of the collection tube volume into the collection tube, cover the collection tube cap, and shake for 4 minutes, open the cap, and discard the xylene;

[0041] (2) Add suspension A accounting for 1 / 2 of the volume of the collection tube to the collection tube described in step (1), cover the tube cap, and shake for 3 minutes, open the tube cap, and empty the collection tube; Suspension A It is made by mixing hydrochloric acid, alcohol and EDTA, and adding 50mg of silicon dioxide particles with a diameter of less than 0.1mm; the concentration of hydrochloric acid is 1.5%; the concentration of alcohol is 30%; the concentration of EDTA is 0.25mol / L; the volume ratio of hydrochloric acid, alcohol and EDTA is 0.5:97:2.

[0042] (3) Add double-distilled water accounting for 1 / 2 of the volume of the collection tu...

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Abstract

The invention discloses a method for cleaning and reusing microdissection sample-collecting tubes. The method can be implemented according to the following steps in turn: (1) adding xylene to a collecting tube, capping and oscillating the collecting tube and discarding the xylene; (2) adding suspension A to the collecting tube, oscillating and then emptying the collecting tube; (3) adding double-distilled water to the collecting tube while adding silica particles, oscillating and then emptying the collecting tube; (4) repeating the step (2) and the step (3) twice in turn; (5) adopting the double-distilled water to wash the collecting tube; (6) uncapping the collecting tube and placing the collecting tube in a refrigerator till liquid completely evaporates; (7) taking the collecting tube out, smearing polylysine on the surface of a viscous module on the inner surface of a tube cap, putting the collecting tube back into the refrigerator and keeping the collecting tube standing; and (8) repeating the step (7). The method can effectively maintain and repair the adhesion property of the viscous module on the inner surface of the tube cap of the collecting tube, and can effectively remove superfluous substances remaining in the collecting tube so as to repeatedly recycle the collecting tube and save a large amount of experiment funding.

Description

technical field [0001] The invention relates to the technical field of cleaning microdissection specimen consumables, in particular to a method for cleaning and reusing microdissection specimen collection tubes. Background technique [0002] The emergence of laser capture microdissection (Laser Capture Microdissection) technology and its system enables relevant scientific researchers to accurately separate target tissue samples, cells (living cells cultured in vitro and tissue cells fixed in slices), organelles and even Chromosomal bands are used for follow-up research, and the accuracy of its control samples is favored by scientific researchers all over the world. At present, most powerful research institutions have been equipped with this technology and related facilities. [0003] When tissue slices are used for microdissection, a special consumable is required. The consumable is a tubular container with a cover (hereinafter referred to as the collection tube). The membr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/06B08B9/08B08B3/12C11D7/60
Inventor 齐瑞群高兴华陈洪铎
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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