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Novel gene combination for detecting colorectal cancer and gene chip thereof

A colorectal cancer and primer combination technology is applied in the field of biological genes to achieve the effects of high sensitivity and accuracy, good specificity, rapid detection and prognosis monitoring

Active Publication Date: 2012-01-04
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]The gene chip originated in the 1980s and has developed rapidly in recent years, but it is mainly used in the fields of medicine and research, and it is used in the early detection of colorectal cancer in my country This is the first time, which will greatly promote the development of early diagnosis of colorectal cancer

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  • Novel gene combination for detecting colorectal cancer and gene chip thereof
  • Novel gene combination for detecting colorectal cancer and gene chip thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Gene Chips Used to Detect Colorectal Cancer Gene Combinations

[0038] 1. Materials and methods

[0039] 1.1 Materials

[0040] 1.1.1 Reagents

[0041] Main reagents RNA extraction reagent (Trizol Reagent), saturated phenol, DEPC, DNA marker, Oligod (T) 18 were purchased from Shanghai Sangong. Reverse transcriptase M-MLV (RNase H-), Taq enzyme, Agarose Gel, M-MLV Rtase cDNA synthesis Kit (code D6130), RNase inhibitor, and agarose (Agarose) were all purchased from Dalian Bao Biological Company. DNA purification kit was purchased from Shanghai Huashun Bioengineering Company; DIG DNA labeling kit and digoxin hybridization detection kit were purchased from Shenzhen Enuojin Biotechnology Co., Ltd.; primers were synthesized by Shanghai Sangon Bioengineering Company; others Reagents were all domestic analytically pure.

[0042] 1.1.2 Clinical data

[0043] (1) Fresh tissue samples of colorectal cancer: The tissue samples were obtained from fresh c...

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Abstract

The invention relates to a gene combination for detecting colorectal cancer. The gene combination comprises 30 gene segments, wherein the 30th gene segment is a quality control gene. The invention also provides a gene chip applying the gene combination. In the prior art, only one or a few of indexes are detected in the detection of the colorectal cancer so as to have the defects of poor specificity, low sensitivity and low accuracy. In the invention, 30 gene segments are designed aiming at 30 indexes of the colorectal cancer and are together used for detecting the colorectal cancer, and a gene chip technology is applied. The invention also provides a gene chip for detecting the colorectal cancer with good specificity, high sensitivity and high accuracy.

Description

technical field [0001] The invention relates to the field of biological genes, in particular to a gene and a gene chip for detecting colorectal cancer. Background technique [0002] Colorectal cancer is one of the common malignant tumors, ranking fifth in the incidence of malignant tumors in my country, and showing three new characteristics: "increasing incidence, earlier age of onset, and high rate of misdiagnosis". In recent years, the incidence of colorectal cancer has increased year by year, from the sixth in the 1970s to the third in the 1990s, and the age of onset tends to be younger. Currently, there are many methods for diagnosing colorectal cancer: CEA (carcinoembryonic antigen) as a clinical monitoring value for colorectal tumor recurrence is far greater than the value of early diagnosis, because it is also highly expressed in other tumors and lacks specificity; Occult blood test (RPHA-FOBT) is still an important means to detect early colorectal cancer, but becau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 车团结徐进章李琳陈小兰
Owner LANZHOU BAIYUAN GENE TECH