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DNA probe, gene chip and application thereof for detecting endometrial cancer

A DNA probe, endometrial cancer technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial measurement / inspection, etc., to achieve high sensitivity and accuracy, high accuracy, and good specificity

Active Publication Date: 2017-07-25
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The gene chip originated in the 1980s and has developed rapidly in recent years, but it is mainly used in the fields of medicine and research. It is the first time that it is used in the early detection of endometrial cancer in my country, which will greatly promote the development of endometrial cancer. Development of Early Cancer Diagnosis

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  • DNA probe, gene chip and application thereof for detecting endometrial cancer
  • DNA probe, gene chip and application thereof for detecting endometrial cancer
  • DNA probe, gene chip and application thereof for detecting endometrial cancer

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Embodiment 1

[0045] 1. Materials and methods

[0046] 1.1 Materials

[0047] 1.1.1 Reagents

[0048] Main reagents RNA extraction reagent (Trizol Reagent), saturated phenol, DEPC, DNA marker, Oligod (T) 18 were purchased from Shanghai Sangong. Reverse transcriptase M-MLV (RNase H-), Taq enzyme, Agarose Gel, RNase inhibitor, and agarose (Agarose) were all purchased from Dalian Bao Biological Company. DNA purification kit was purchased from Shanghai Huashun Bioengineering Company; DIG DNA labeling kit and digoxin hybridization detection kit were purchased from Shenzhen Enuojin Biotechnology Co., Ltd.; primers were synthesized by Shanghai Sangon Bioengineering Company; others Reagents were all domestic analytically pure.

[0049] Preparation of prehybridization solution: (6×SSC, 5×Denhardt’s reagent, 0.5% SDS, 50% (v / v) formamide, ddH 2 O, 100 μg / ml salmon essence was added after DNA denaturation. Note: 0.2ml of pre-hybridization solution is required for each square centimeter of nylon m...

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Abstract

The invention belongs to the field of biotechnology, and discloses a DNA probe for detecting endometrial carcinoma. The DNA probe for detecting endometrial carcinoma has a nucleotide sequence shown by sequence tables from SEQ ID No.1 to 26, and has strong specificity and sensitivity for detecting endometrial carcinoma. The invention also provides a gene chip comprising the DNA probe. By adopting the gene chip provided by the invention, the high-flux, automatic and quick detection of a sample probably having endometrial carcinoma can be realized, and the gene chip has strong specificity, can ensure the accuracy of a detection result and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a DNA probe, a gene chip and an application thereof for detecting endometrial cancer. Background technique [0002] Endometrial cancer, also known as uterine body cancer, refers to a group of epithelial malignant tumors that originate in the endometrium, most of which originate in endometrial glands, and are called endometrial adenocarcinoma or endometrioid adenocarcinoma. It is one of the three most common malignant tumors of the female reproductive tract, accounting for 7% of female systemic malignant tumors and 20% to 30% of female reproductive tract malignant tumors. It is more common in elderly women. In recent years, the incidence rate has been increasing worldwide, second only to cervical cancer, but the 5-year survival rate has been decreasing year by year. The diagnosis and clinical staging of endometrial cancer are mainly determined based on the results of medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C40B40/06
CPCC12Q1/6886C12Q2600/166
Inventor 车团结文惠方陈小兰李琳张尚弟蔡传勇
Owner LANZHOU BAIYUAN GENE TECH