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Novel gene combination for detecting colorectal cancer and gene chip thereof

A technology of gene combination and gene chip is applied in the field of colorectal cancer detection gene and its gene chip to achieve the effects of good specificity, avoiding wrong diagnosis, high sensitivity and accuracy

Active Publication Date: 2010-01-13
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]The gene chip originated in the 1980s and has developed rapidly in recent years, but it is mainly used in the fields of medicine and research, and it is used in the early detection of colorectal cancer in my country This is the first time, which will greatly promote the development of early diagnosis of colorectal cancer

Method used

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  • Novel gene combination for detecting colorectal cancer and gene chip thereof
  • Novel gene combination for detecting colorectal cancer and gene chip thereof
  • Novel gene combination for detecting colorectal cancer and gene chip thereof

Examples

Experimental program
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Embodiment 1

[0033] Example 1 Preparation of a gene chip using a new gene combination for the detection of colorectal cancer

[0034] 1. Materials and methods

[0035] 1.1 Materials

[0036] 1.1.1 Reagents

[0037] Main reagents RNA extraction reagent (Trizol Reagent), saturated phenol, DEPC, DNA marker, Oligod (T) 18 were purchased from Shanghai Sangong. Reverse transcriptase M-MLV (RNase H-), Taq enzyme, Agarose Gel, M-MLV Rtase cDNA synthesis Kit (code D6130), RNase inhibitor, and agarose (Agarose) were all purchased from Dalian Bao Biological Company. DNA purification kit was purchased from Shanghai Huashun Bioengineering Company; DIG DNA Labeling Kit and Digoxin Hybridization Detection Kit were purchased from Shenzhen Enokin Biotechnology Co., Ltd.; primers were synthesized by Shanghai Sangon Bioengineering Company; other All reagents were domestic analytical grade.

[0038] 1.1.2 Clinical data

[0039] (1) Fresh tissue samples of colorectal cancer: Tissue samples were obtained f...

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Abstract

The invention relates to a gene combination for detecting colorectal cancer. The gene combination comprises 30 gene segments, wherein the 30th gene segment is a quality control gene. The invention also provides a gene chip applying the novel gene combination. In the prior art, only one or a few of indexes are detected in the detection of the colorectal cancer so as to have the defects of poor specificity, low sensitivity and low accuracy. In the invention, 30 gene segments are designed aiming at 30 indexes of the colorectal cancer and are together used for detecting the colorectal cancer, and a gene chip technology is applied. The invention also provides a gene chip for detecting the colorectal cancer with good specificity, high sensitivity and high accuracy.

Description

technical field [0001] The invention relates to the field of biological genes, in particular to a gene used for colorectal cancer detection and a gene chip thereof. Background technique [0002] Colorectal cancer is one of the common malignant tumors, ranking fifth in the incidence of malignant tumors in my country, and showing three new characteristics of "rising incidence, early age of onset, and high misdiagnosis rate". In recent years, the incidence of colorectal cancer has increased year by year, from the sixth in the 1970s to the third in the 1990s, and the age of onset tends to be younger. At present, there are various methods for diagnosing colorectal cancer: CEA (carcinoembryonic antigen) as the clinical monitoring value of colorectal tumor recurrence is far greater than the value of early diagnosis, because it is also highly expressed in other tumors and lacks specificity; stool Occult blood test (RPHA-FOBT) is still an important method to detect early colorectal ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 车团结徐进章李琳陈小兰
Owner LANZHOU BAIYUAN GENE TECH