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Spittle rapid detection strip for chlamydia trachomatis antibody

A technology for detecting Chlamydia trachomatis and test strips, applied in the field of biological diagnosis, can solve the problems of high detection cost and unsuitability for on-site detection, and achieve high sensitivity, suitable for on-site detection and self-inspection, and fast response

Inactive Publication Date: 2010-03-03
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a saliva rapid detection test strip for Chlamydia trachomatis antibody, which can use saliva as a sample, and solves the problem that the existing test strips are not suitable for on-site detection and the detection cost is high

Method used

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  • Spittle rapid detection strip for chlamydia trachomatis antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of colloidal gold pads

[0024] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 0.75 mg of a mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the Chlamydia trachomatis antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin ( BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0025] Coating of nitrocellulose membrane

[0026] The Chlamydia trachomatis antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to t...

Embodiment 2

[0031] Preparation of colloidal gold pads

[0032] Prepare a colloidal gold solution with a diameter of 35nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.2, add 1.3 mg of a mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the Chlamydia trachomatis antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin ( BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0033] Coating of nitrocellulose membrane

[0034] The Chlamydia trachomatis antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to th...

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Abstract

The invention discloses a spittle rapid detection strip for a chlamydia trachomatis antibody, comprising a baseboard, to which a sample pad, a colloidal gold pad, a nitrocellulose membrane and a sample-drawing pad are sequentially adhered, wherein a mouse-anti-human IgG antibody which is marked by the colloidal gold and can be integrated with the specificity of a chlamydia trachomatis antibody isattached to the colloidal gold pad; and the nitrocellulose membrane is coated with a detection line formed by the chlamydia trachomatis antibody and a quality control line formed by the goat-anti-mouse antiantibody which can be integrated with the specificity of mouse-anti-human IgG antibody. The spittle rapid detection strip provided by the invention adopts the colloidal gold marking technology to detect the chlamydia trachomatis antibody in spittle so as to determine whether the person is infected with chlamydia trachomatis, and has the advantages that operation is simple; reaction is quick;sensitivity is high; specificity is strong; and the spittle rapid detection strip can be applied to both on-site detection and self-detection, and is economical and practical and the like.

Description

technical field [0001] The invention relates to the technical field of biological diagnosis, in particular to a saliva rapid detection test strip for Chlamydia trachomatis antibody. Background technique [0002] "Berger's System Handbook" (1984) records that Chlamydiatrachomatis is divided into three biotypes, namely, mouse biotype (biovar mouse), trachoma biotype (biovar trachoma) and venereal disease lymphogranuloma biotype. The latter two are associated with human disease. Using indirect micro-immunofluorescence test, trachoma biotypes are divided into A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K14 serotypes, and LGV biotypes are further divided into L1, L2, L2a, L34 serotypes. [0003] Urogenital tract infections are transmitted by sexual contact and are caused by trachoma biological variants D-K serotypes. Males often present with urethritis, which can be relieved without treatment, but most of them become chronic, with periodic aggravation, and can be combined with e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/532G01N33/52
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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