Transgenic plants with increased stress tolerance and yield

A technology of transgenic plants and plants, applied in plant peptides, plant products, genetic engineering, etc., can solve problems such as weight gain

Inactive Publication Date: 2010-03-24
BASF PLANT SCI GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Larger plants with greater leaf area are generally able to absorb more light and carbon dioxide than smaller plants and are therefore likely to gain more weight in the same time period

Method used

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  • Transgenic plants with increased stress tolerance and yield
  • Transgenic plants with increased stress tolerance and yield
  • Transgenic plants with increased stress tolerance and yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Identification of open reading frames in Physcomitrella patens

[0080] A cDNA library prepared from the plant species Physcomitrella patens (Hedw.) B.S.G. from the collection of the Department of Genetic Research of the University of Hamburg was sequenced using standard methods. The plants were originally derived from strain 16 / 14 in the H.L.K. Whitehouse collection at Gransden Wood, Huntingdon (UK), subcultured from spores by Engel (1968, Am. J. Bot. 55:438-446).

[0081] Partial cDNA (EST) of Physcomitrella patens was identified using the EST-MAX program (Bio-Max (Munich, Germany)) in the Physcomitrella patens EST sequencing project. The full-length nucleotide cDNA sequence was determined using known methods. Amino acid sequence identities and similarities of the disclosed polypeptide sequences to known protein sequences are shown in Tables 2-5 (pairwise comparison used with Align and default settings).

[0082] Table 2

[0083] Comparison of EST462 (SEQ ID NO: 2)...

Embodiment 2

[0095] Cloning full-length cDNA from other plants

[0096] Canola, soybean, rice, maize, linseed, and wheat plants were grown under various conditions and treatments, and different tissues were harvested at various developmental stages. Plant growth and harvesting is performed in a strategic manner to maximize the probability of harvesting all expressible genes in at least one or more of the generated libraries. mRNA was isolated from each pooled sample, and a cDNA library was constructed. No amplification step was applied during library preparation to minimize gene redundancy within samples and preserve expression information. All libraries were generated from 3' from mRNA purified on oligo-dT columns. Colonies obtained by transforming Escherichia coli with the cDNA library were randomly picked and placed in microtiter plates.

[0097] Plasmid DNA was isolated from E. coli colonies and blotted onto membranes. A group of 288 species 33 P radiolabeled 7-membered (mer) olig...

Embodiment 3

[0205] Stress tolerant Arabidopsis plants

[0206] The fragment containing the Physcomitrella patens polynucleotide was ligated into a binary vector containing a selectable marker gene. The resulting recombinant vector contains the corresponding gene in sense orientation under the control of a constitutive superpromoter. The recombinant vectors were transformed into Agrobacterium tumefaciens C58C1 and PMP90 plants according to standard conditions. Arabidopsis ecotype C24 plants were grown and transformed according to standard conditions. T1 plants are screened for resistance to the selection agent conferred by the selectable marker gene, and T1 seeds are collected.

[0207] Physcomitrella patens polynucleotides are overexpressed in Arabidopsis under the control of a constitutive promoter. T2 and / or T3 seeds are screened in plates for resistance to the selection agent conferred by the selectable marker gene, and positive plants are transplanted into soil and grown in a growt...

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Abstract

Polynucleotides are disclosed which are capable of enhancing a growth, yield under water- limited conditions, and / or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Description

[0001] This application claims the benefit of priority to US Provisional Patent Application Serial No. 60 / 932,147, filed May 29, 2007, the contents of which are incorporated herein by reference. technical field [0002] The present invention generally relates to transgenic plants overexpressing a nucleic acid sequence encoding a polypeptide capable of conferring increased stress tolerance and thus increased plant growth and crop yield under normal or abiotic stress conditions. In addition, the present invention relates to novel isolated nucleic acid sequences encoding the ability to confer on plants increased stress tolerance under abiotic stress conditions, and / or increased plant growth and / or increased plant growth under normal or abiotic stress conditions. Peptides for Crop Yield. Background technique [0003] Abiotic environmental stresses such as drought, salinity, heat and cold are major limiting factors for plant growth and crop yield. Crop yield is defined herein as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC07K14/415C12N15/8273C12N15/8261Y02A40/146
Inventor D·艾伦A·舍利B·麦克尔西
Owner BASF PLANT SCI GMBH
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