Preparation method and application of flavone and flavan derivatives

A compound and chelate technology, which is applied in the field of preparation and application of flavonoids and flavan derivatives, can solve the problems that the anti-hypoxic activity of flavonoids and flavan compounds has not been reported, and the anti-hypoxic active ingredients have not been clarified.

Inactive Publication Date: 2010-03-31
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the hypoxic protective effect of total flavonoids of South Suanzizao fruit has also been documented (Li Zengxi et al.; Protective effect of total flavonoids of Guangzao jujube on animal hypoxia tolerance and acute myocardial ischemia: Chinese Herbal Medici

Method used

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  • Preparation method and application of flavone and flavan derivatives
  • Preparation method and application of flavone and flavan derivatives
  • Preparation method and application of flavone and flavan derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1. The separation and preparation of compound I

[0032] 3.2kg of dry bark of Choerospondias axillaries (Roxb.) Burtt. et Hill. (collected in August 1999 from Mengla, Yunnan) was pulverized and extracted with commercially available medical ethanol at room temperature, each time with 95% (V / V ) in 25 liters of aqueous ethanol soaked for 7 days, extracted 4 times altogether, combined extracts, concentrated and dried under reduced pressure to obtain 750 g of ethanol extract. 750 g of the ethanol extract was suspended in 3 liters of water, extracted successively with chloroform, ethyl acetate, and n-butanol, each solvent was used for 3 liters each, extracted 4 times respectively, the same extracts were combined, concentrated and dried under reduced pressure, and obtained 60 g of the chloroform extract, 310 g of the ethyl acetate extract, 300 g of the n-butanol extract, and 80 g of the aqueous layer residue.

[0033] Take 100g of ethyl acetate extract, dissolve it ...

Embodiment 2

[0036] Embodiment 2. The separation and preparation of compound II and III

[0037] Dissolve 300 g of the n-butanol extract of Suanzi jujube obtained in Example 1 with an appropriate amount of methanol, add an appropriate amount of macroporous resin AB-8 for adsorption, dry, and put on the macroporous resin AB-8 column (column bed in water 8.5cm × 48cm), Gradient elution with water-ethanol (100:0 → 0:100) in different volume ratios, the combined eluents were collected according to the TLC test results, concentrated and dried under reduced pressure, and two water-washed fractions B- 1 (20 g), B-2 (38 g) and several subsequent eluting aqueous ethanol fractions B-3 (total 230 g, water-ethanol 90:10→0:100 elution fraction).

[0038] Component B-2 (38g) was dissolved in an appropriate amount of methanol, added an appropriate amount of polyamide for adsorption, dried, and applied to a polyamide column (column bed in water 7.5cm×18.5cm), and eluted with a gradient of water-acetone in...

Embodiment 3

[0042] Embodiment 3. The separation and preparation of compound IV and V

[0043] The medicinal dregs extracted by volume percentage 95% ethanol in Example 1 are further extracted with 60% volume percentage ethanol water at room temperature, soaked for 7 days with 25 liters of aqueous ethanol each time, and are extracted 3 times altogether, and the combined extracts are reduced. Concentrate and dry under pressure to obtain 90 g of aqueous ethanol extract.

[0044] Take 80g of the hydrous ethanol extraction extract, dissolve it with an appropriate amount of methanol, add 150g of polyamide to absorb, dry, put on a polyamide column (fill 250g of polyamide in water, column bed 8.5cm×22cm), and elute with water-ethanol-acetone system , according to the thin layer detection results, the combined eluate was collected, concentrated and dried under reduced pressure to obtain five components: WE-1 (10g, water eluted part), WE-2 (17g, water eluted part), WE-3 (2g, water-ethanol volume rat...

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Abstract

The invention relates to flavone and flavan derivatives contained in medical plant choerospondias axillaries, a preparation method thereof, and an application of the compounds in preparing antihypoxicdrugs, antihypoxic health products and other functional foods.

Description

Technical field: [0001] The invention relates to a preparation method of flavonoids and flavan derivatives contained in the medicinal plant Jujube jujuba, and the use of these compounds for preparing functional foods such as anti-hypoxia drugs and anti-hypoxia health products. Background technique: [0002] Oxygen is indispensable in human life activities, and oxygen molecules play an important role in the energy metabolism of tissues and cells in the body. A variety of factors can lead to insufficient oxygen supply to human tissues and cells, such as certain diseases or being in a special hypoxic environment (such as plateau), etc., and hypoxia can in turn cause cell and tissue damage and even various serious diseases, such as Severe respiratory diseases, concurrent cardiovascular and cerebrovascular diseases, and low immune function. Severe hypoxia is often the direct cause of death in many related critically ill patients. Therefore, the research and development of anti-...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/352A61P43/00C07H17/07C07H15/26C07D311/62C07D493/04A23L1/30A23L33/105
Inventor 崔承彬李长伟蔡兵韩冰李明明范明
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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