Method for improving specificity in cutting position of endonuclease V

An endonuclease and specific technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as errors and sequencing speed limitations, and achieve high cutting efficiency, increased read length, and less impact Effect
CN101693918BInactive Publication Date: 2012-01-04SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2012-01-04
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The method provides a method for improving the specificity in a cutting position of endonuclease V and relates to a high-throughput method for analyzing DNA sequences, in particular to a high-speed low-cost and high-throughput method for DNA sequencing. Recognition-site deoxyinosine dI containing the endonuclease V and DNA segments of single-chain oligonucleotide at a site where oxygen on one or more phosphodiester bonds in a DNA chain is sulfo-modified are introduced into a DNA probe, and the distance between the recognition site of the endonuclease V and the sulfo-modified site is two or more basic groups. The method can be used for analyzing unknown DNA, namely sequence information of a template DNA to be analyzed. Re-hybridized high-throughput primers are obtained by synthesis and purification by a very mature solid-phase DNA method, and therefore, the method does not have wrongly extended accumulative effect and can maintain the amount of DNA templates and sequencing primers. Thedetermination of the sequence is correct and reliable, having no limitations on sequenced length.
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Description

technical field

[0001] The invention relates to a high-throughput DNA sequence analysis method, in particular to a fast, low-cost and high-throughput DNA sequence method. Belongs to the field of biotechnology. Background technique

[0002] Nucleic acid (such as DNA) contains the blueprint of all living organisms, and the study of nucleic acid sequences is crucial to life sciences. With the development and completion of the Human Genome Project and various model organism genome projects, human beings have entered the post-gene era, which has had a huge impact on contemporary biological research and medical research, and the related disciplines of molecular biology have been rapidly developed. develop. It will become possible to understand the differences of life, the law of disease occurrence and development, and the interaction between drugs and living organisms at the genetic level. As far as gene sequence analysis is concerned, the focus of the post-genome era has shift...

Claims

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