The method of adding metal ions to increase the output of griseovirmycin a

A technique for adding griseocyanin and metal, which is applied in the field of increasing the yield of griseogreenin A and realizing the increase of the yield of griseocyanin A, and can solve the problems of low fermentative yield of griseocyanin A and the like.

Inactive Publication Date: 2011-12-21
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the defect of low fermentation yield of Giseoviridin A, and provide a method for increasing Giseoviridin A yield in two different culture media by adding heavy metal ions

Method used

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  • The method of adding metal ions to increase the output of griseovirmycin a
  • The method of adding metal ions to increase the output of griseovirmycin a
  • The method of adding metal ions to increase the output of griseovirmycin a

Examples

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Effect test

Embodiment 1

[0052] Example 1 Effect of adding cobalt nitrate hexahydrate on the synthesis of griseoviridin A and the growth of bacteria in the fermentation medium A

[0053] Such as figure 1 As shown, when cultivating Aspergillus cinerea HB1-19 in fermentation medium A, by adding 7.5mg / l, 15mg / l, 30mg / l final concentration of cobalt nitrate hexahydrate, investigate its synthesis of griseovirmycin A and Effects on the growth of Aspergillus aeruginosa. As a result, it was found that cobalt nitrate hexahydrate with a final concentration of 7.5 mg / l was 29% higher than the control group without any additives; Synthetic promoting effect is the largest, can make its output reach 24.3mg / l, than the control group without any additives has increased by 67%; and when adding the cobalt nitrate hexahydrate that final concentration is 30mg / l, the output of griseovirmycin A Only a 41% improvement over the control group. And the addition of this substance has almost no effect on the growth of Aspergi...

Embodiment 2

[0054] Example 2 Effects of different addition times and time of cobalt nitrate hexahydrate with a final concentration of 15 mg / l in fermentation medium A

[0055] Such as figure 2 As shown, when Aspergillus aeruginosa HB1-19 was cultivated in fermentation medium A, the optimal number of additions was determined by adding different times of cobalt nitrate hexahydrate with a final concentration of 15 mg / l. The result shows that when adding the cobalt nitrate hexahydrate at a final concentration of 15mg / l in the fermentation medium A, it increases by 27% compared with the control group; , increased by 39% than the control group without any additives; when adding cobalt nitrate hexahydrate with a final concentration of 15 mg / l in 3 times, the output of griseoviridin A increased by 74% compared with the control group; added in 4 times When the final concentration of cobalt nitrate hexahydrate was 15 mg / l, the output of griseoviridin A was only increased by 48% compared with the ...

Embodiment 3

[0056] Effect of adding nickel chloride on the synthesis of griseovirmycin A and the growth of bacteria in embodiment 3 fermentation medium A

[0057] Such as Figure 4 As shown, when culturing Aspergillus cinerea HB1-19 in fermentation medium A, by adding 7.5mg / l, 15mg / l, 30mg / l final concentration of nickel chloride, study its effect on the production of griseovirmycin A and gray Effects on the growth of Aspergillus aeruginosa. Experimental result shows that final concentration is the nickel chloride of 7.5mg / l, improves 1.15 times than the control group without any additive; The yield of A increased by 44%; when adding nickel chloride with a final concentration of 30mg / l, the yield of griseoviridin A increased by 21% compared with the control group without additives. The addition of nickel chloride had almost no effect on the growth of A. griseus HB1-19. According to the scheme of embodiment 2, it is determined that the optimum addition mode of nickel chloride in ferment...

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Abstract

The invention discloses a method for increasing the yield of griseoviridin A by adding metal ions, which belongs to the technical field of metabolic regulation and optimization of fermentation process. The present invention utilizes the marine filamentous fungus Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) CCTCC M 206022 as the production strain, by adding nickel chloride, cobalt chloride and cobalt nitrate hexahydrate in two different fermentation media To increase the production of griseovirmycin A. Among them, the highest yield in fermentation medium A can reach 24.3mg / l, which is a 67% increase in the control group without adding metal ions; the highest yield in fermentation medium B can reach 81.1mg / l, which is without adding metal ions The control group improved by 45%. The invention is of great significance for the fermentation research of the marine fungus Aspergillus cinerea HB1-19 and the research on the metabolism regulation of griseovirmycin A.

Description

technical field [0001] The invention relates to a method for increasing the yield of griseoviridin A, mainly through adding metal ions (cobalt ions and nickel ions) to realize the method for increasing the yield of griseoviridin A in two different mediums. It belongs to the technical field of metabolic regulation and optimization of fermentation process. Background technique [0002] Giseoviridin A is a kind of independently isolated marine fungus Aspergillus glaucus (Aspergillus glaucus) HB1-19 (preservation date 2006.3.20, China Center for Type Culture Collection, preservation number CCTCCM 206022) by Ocean University of China. Anthraquinone derivatives with intellectual property rights and novel structures have strong anticancer activity. Giseoviridin A uses 9-anthrone as the core, and C-10 and C-4 in the molecule form a six-membered lactone ring. It has been proved by experiments that griseovirmycin A inhibits the proliferation of human lung cancer A549 cells, human li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06C12R1/66
Inventor 周祥山张元兴孙学谦
Owner EAST CHINA UNIV OF SCI & TECH
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