Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof

A technology of wheat bran and polysaccharide, which is applied in the directions of antitumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as few related reports, and achieves low price, broad prospects for industrial development, and sources of rich effects

Active Publication Date: 2010-05-12
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Research and product development in this area are carried out wi

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  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof
  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof
  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof

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[0035] Example 1. Extraction of wheat bran immunomodulation and anti-tumor activity polysaccharides

[0036] 1. Polysaccharide extraction: The flow chart of polysaccharide extraction is as follows figure 1 Shown.

[0037] 1) Wheat bran pretreatment

[0038] Weigh 100 g of wheat bran, disperse it in 500 mL of a dilute hydrochloric acid solution with a pH of 3.5, and soak at 25°C for 12 hours. Centrifugal separation (1500rpm, 30min), collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, and drain the water to obtain dephytic acid and destarch wheat bran.

[0039] 2) Enzymatic hydrolysis

[0040] Disperse the dephytic acid and destarch wheat bran into 400mL water, adjust the pH of the system to 5.5, and add 0.25g xylanase (the ratio of xylanase to wheat bran is 6.25U:1g without Pretreated wheat bran), placed in a 55 ℃ heat preservation tank, stirred and extracted; after reaction for 4.0 h, the enzymatic hydrolysis pro...

Example Embodiment

[0072] Example 2: Extraction of wheat bran immunomodulation and anti-tumor activity polysaccharides

[0073] 1. Polysaccharide extraction

[0074] 1) Wheat bran pretreatment

[0075] Weigh 1 kg of wheat bran, disperse it in 4L of dilute hydrochloric acid solution with pH 3.0, and soak overnight (10 hours) at room temperature (23°C). Centrifugal separation (1500rpm, 30min), collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, and drain the water to obtain desphytic acid and destarch wheat bran.

[0076] 2) Enzymatic hydrolysis

[0077] Disperse the dephytic acid and destarch wheat bran into 6L water, adjust the pH of the system to 5.0, add 4.5g xylanase (the ratio of xylanase to wheat bran is 8U:1g wheat bran ), placed in a 60°C heat preservation tank, stirred and extracted; after reacting for 3.0 hours, the enzymatic hydrolysis product was obtained; the enzymatic hydrolysis product was heated to 95°C, kept for 10 ...

Example Embodiment

[0097] Example 3. Extraction of wheat bran immunomodulation and anti-tumor activity polysaccharides

[0098] 1. Polysaccharide extraction

[0099] 1) Wheat bran pretreatment

[0100] Weigh 5 kg of wheat bran, disperse it in 20L of dilute hydrochloric acid solution with a pH of 3.0, and soak it overnight (14 hours) at room temperature (27°C). Centrifugal separation (1500rpm, 30min), collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, and drain the water to obtain desphytic acid and destarch wheat bran.

[0101] 2) Enzymatic hydrolysis

[0102] Disperse the dephytic acid and destarch wheat bran into 35L water, adjust the pH of the system to 5.3, add 20g xylanase (the ratio of xylanase to wheat bran is 10U:1g wheat bran) , Placed in a 50°C heat preservation tank, stirred and extracted; after reaction for 2.0h, the enzymatic hydrolysis product was obtained; the enzymatic hydrolysis product was heated to 95°C, kept fo...

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Abstract

The invention discloses a wheat bran polysaccharide with antitumor and immune regulation activities and an extraction method thereof. The method for extracting the polysaccharide comprises the following step: performing enzymolysis of wheat bran with xylanase to obtain the polysaccharide, wherein the proportion of xylanase and wheat bran is (5U-12.5U):1g. The method of the invention adopts wheat bran which is a subsidiary agricultural product resource, to largely prepare the polysaccharide product by processing and extracting, the polysaccharide product mainly contains araboxylan (or pentosan); animal experiments show that the polysaccharide has the similar immune regulation and antitumor effects of the antitumor adjuvant drugs which are used in clinic; in addition, the price of raw materials of the polysaccharide is cheap and the sources are wide so that the polysaccharide of the invention has more market competition advantage and wide industrialized development prospect.

Description

technical field [0001] The invention relates to a wheat bran polysaccharide with antitumor and immune regulation activities and an extraction method thereof. Background technique [0002] Wheat bran is a by-product of wheat processing, mainly composed of wheat seed coat, aleurone layer and residual endosperm. Wheat bran is a typical grain dietary fiber raw material. Its composition is complex. In addition to the main cell wall constituent arabinoxylan (or pentosan), there are also cellulose, lignin, dextran, phytic acid, and starch. Wait. At present, in our country, most of wheat bran is used as feed for livestock and poultry and microbial fermentation medium. The high added value and in-depth research and development mainly focus on using it as a dietary fiber raw material to exert physiological functions such as laxative and blood fat reduction. , or as a dough improver or as an application function of edible glue. [0003] In domestic patents, wheat bran is mostly used...

Claims

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Application Information

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IPC IPC(8): C12P19/14C08B37/14A61K31/715A61P37/04A61P35/00A61K36/899
Inventor 周素梅曹丽王强刘红芝张晓娜任霞
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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