The application provides a koji making method for improving
protease content, and belongs to the technical field of condiment manufacturing. The koji making method for improving
protease content comprises the following steps: preparing
protein soybean raw materials and
starch wheat raw materials, inoculating
Aspergillus oryzae, mixing and stirring, and then carrying out koji making; the koji making is divided into a first culture stage, a second culture stage, a third culture stage and a fourth culture stage according to koji making time; the first culture stage is 0-13 hours of koji making; the second culture stage is 14-21 hours of koji making; the third culture stage is 22-30 hours of koji making; and the fourth culture stage is 31-44 hours of koji making. The application mainly designs
oxygen demand of
Aspergillus oryzae at different growth stages, combines heat production rules of
Aspergillus oryzae
metabolism, and creatively realizes optimization combination of circulating air and
fresh air from outside and optimization of
air volume at different stages, so that the koji quality is improved.