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Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof

A MFG-E8, single nucleotide polymorphism technology, applied in the field of molecular genetics, can solve problems such as lack of research

Inactive Publication Date: 2010-05-12
NORTHWEST A & F UNIV
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Problems solved by technology

At present, research on the genetic variation of the MFG-E8 gene in Chinese dairy goats is lacking, and research on the function of the gene locus and its association with economic traits (such as body height, body length, milk production, milk composition, etc.) is still lacking. is blank

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  • Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof
  • Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof
  • Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof

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Embodiment Construction

[0030] The present invention uses the RFLP-PCR method to detect the single nucleotide polymorphism that may result in a change in the expression level of the encoded protein due to the mutation of the 12892bp synonymous codon of the dairy goat MFG-E8 gene. The present invention will be further described in detail below. What has been described is by way of explanation, not limitation, of the invention.

[0031] a, Dairy goat MFG-E8 gene contains the design of the PCR primer of the seventh exon region

[0032] The present invention is based on the sequence of the homologous animal bovine of dairy goat, specifically the sequence of Angus cattle (NC_007319) published by NCBI; using Primer 5.0 to design the seventh gene containing dairy goat MFG-E8 Exon PCR primers, the primer sequences are as follows:

[0033] Upstream primer: tcttttccct tcactgcctc 20;

[0034] Downstream primer: acaaggctca aagaaactcc 20;

[0035] Using the above primers to amplify the dairy goat genome, a 397...

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Abstract

The invention discloses a mononucleotide polymorphism of milk goat MFG-E8 genes and a detection method thereof. The invention takes the whole genome DNA of the milk goat to be detected containing the MFG-E8 genes as the template, takes the primer coupled P (comprising P1, R1 and newP) as the primer, and takes the PCR amplified milk goat MFG-E8 as the genes; the restriction enzyme EcoRV is utilized to digest PCR amplifying products, and then polyacrylamide gel electrophoresis is carried out on the amplified segments after enzyme digestion; the mononucleotide polymorphism of the 12892nt of the milk goat MFG-E8 genes is identified according to the results of polyacrylamide gel electrophoresis. As the MFG-E8 gene function relates to development of breast, butter fat, total solids and other milk elements, the detection method provided by the invention lays a foundation for the establishment of the relation between the SNP and the milk component of the MFG-E8 genes, so as to be used for the marker-assisted selection of the properties of Chinese milk goat and the rapid establishment of milk goat species group with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a single nucleotide polymorphism (SNP) of a functional gene of a dairy goat as a molecular genetic marker, in particular to a single nucleotide polymorphism of a dairy goat MFG-E8 gene and its detection method. Background technique [0002] Goat milk has high nutritional value, does not contain allergens, has balanced nutritional components, is easy to digest and absorb, and is similar to human milk in protein composition; the volume of fat particles in goat milk is one-third that of milk, and the protein, minerals The content of substances, especially calcium, phosphorus, vitamins and trace elements is slightly higher than that of milk, and it is more conducive to human body absorption. It is known as the "king of milk" in the international nutrition circle. Fewer people drink goat's milk than goat's milk because of its smell and taste. With the application of high-tech in biop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏屈玉娇蓝贤勇刘艳丽李转见陈忠琦淮永涛雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
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