Special probe and gene chip used for identifying pathogenic aspergillus
A technology for pathogenic Aspergillus and Aspergillus, which is applied in the field of special probes and gene chips for identifying pathogenic Aspergillus, and can solve the problems of not being able to specifically detect pathogenic Aspergillus
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Embodiment 1
[0043] Example 1. Detection of pathogenic Aspergillus and rapid identification of strains
[0044] Extract the genomic DNA from the samples containing the pathogenic Aspergillus of different known strains according to the standard method, and then use the above primer pair (sequence 11 and 12 in the sequence list) to perform PCR reaction (about 1.5 hours), and then the PCR product With the nitrocellulose membranes labeled with different probes (respectively the probes shown in sequence 1 to sequence 10), the flow-through hybridization reaction is carried out through the medical nucleic acid molecular hybridization instrument, and the bacterial species in the tested sample are judged according to the result of the reaction. The time required is 3.5 hours. The results are shown in Table 1.
[0045] Table 1 Identification results of common pathogenic Aspergillus
[0046] sample
Embodiment 2
[0047] Example 2: Detection of pathogenic Aspergillus mixed bacteria and rapid identification of strains
[0048] A sample containing a mixture of known pathogenic Aspergillus strains was extracted according to standard methods to extract genomic DNA, and then the primer pair (sequence 11 and 12 in the sequence listing) was used for PCR reaction (about 1.5 hours), and then the PCR product was combined with The nitrocellulose membranes labeled with different probes (respectively the probes shown in sequence 1 to sequence 10) are subjected to a flow-through hybridization reaction (about 2 hours) through a medical nucleic acid molecular hybridization instrument, and the test sample can be judged according to the result of the reaction The total time required for the mixed strains is 3.5 hours. The results are shown in Table 2.
[0049] Table 2 Identification results of mixed samples of pathogenic Aspergillus
[0050] specimen
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