Method for Flow Cytometry Sorting of Cells Using Miniature Light Patterns
A cell and pattern technology, applied in the field of microfluidics and microfluidics biomedical analysis chips, can solve the problems of complex microelectrode structure, complex chip structure, high production cost, etc., to achieve flexible and efficient separation, improve efficiency, and reduce costs Effect
Inactive Publication Date: 2012-02-22
SOUTHEAST UNIV
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[0005] Technical problem: The purpose of the present invention is to provide a method for flow counting and sorting cells using microscopic light patterns, so as to solve the problem of high cost of conventional flow analyzers and the conventional biological cell flow continuous micro-analysis chip microelectrodes. Defects with complex structure or chip structure and high production cost
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[0021] For an example of the method for performing flow counting and sorting of cells using microphotographic patterns provided by the present invention, see figure 1 with figure 2 . The chip structure, material, and light pattern pattern that can be used in this method are not limited to this embodiment. The cell counting and sorting chip used in this embodiment includes a sample inlet 11, a transparent insulating cover sheet 12, an upper transparent indium tin oxide film 13, an intermediate spacer layer 2, a microfluidic cavity 21, and a transparent insulating substrate 34, A light pattern dummy electrode forming layer composed of a silicon nitride layer 31, a photoconductive layer 32 and a transparent conductive layer 33. The silicon nitride layer 31 can prevent hydrolysis, and the photoconductive layer 32 has the characteristics of photoconductivity, that is, the number of internal carriers increases sharply when illuminated by light, and the number of internal carriers i...
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The method of counting and sorting cells by using microscopic light patterns is to sequentially construct light patterns to form cell convergence channels, single cell sampling identification channels, and cell separation channels, thereby realizing continuous cell convergence, identification, and separation. During the identification process, the classification and counting of various cells is completed at the same time, and the sorting microsystem composed of the above three channels in turn finally realizes the counting and sorting of various cells in biological samples. The method for counting and separating biological cells provided by the present invention makes full use of the flexibility advantages of microscopic light patterns, and at the same time avoids making complex physical entity electrode arrays on the chip, and is superior to current biological cells in terms of cost, function and performance. counting sorting method.
Description
Technical field [0001] The invention is a method for implementing flow counting and sorting of biological cells by using a photoelectric microfluidic device, and relates to the field of microfluidics, especially the field of microfluidic biomedical analysis chips. Background technique [0002] The traditional flow cytometer has been widely used in the medical industry and biomedical research institutions. It mainly converges the cells into a single cell queue, and makes the cells pass through a flow detector in sequence. At this time, the cells are in a narrow bundle. In the focused light path, each cell will block the light beam once when passing through, and a certain number of cells will continuously interrupt the light beam, so that the detector can detect the number of light blocking times per unit time, thereby calculating the number of cells. The light scattering method commonly used in detection can also judge the volume and shape of each cell based on the scattering angl...
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IPC IPC(8): G01N1/14B01L3/00
Inventor 常严朱晓璐易红倪中华
Owner SOUTHEAST UNIV