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Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof

A oat acid bacteria, biodegradation technology, applied in the directions of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of not being degraded, unable to effectively remove estrogen, etc., and achieve the effect of increasing the rate

Inactive Publication Date: 2010-07-28
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

Conventional active sewage treatment systems cannot effectively remove estrogen in raw water. Although estrone and 17β-estradiol can be partially degraded, 17α-ethinyl estradiol is basically not degraded, resulting in ng / L Levels of estrogen were detected (Ternes et al., Sci.Total.Environ.Vol.225, 91-99, 1999)

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  • Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof
  • Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof
  • Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof

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Embodiment Construction

[0015] 1. Isolation, screening and preservation of the Acidovorax avenae SS-1 strain provided by the present invention

[0016] The activated sludge in the denitrification and phosphorus removal process system of the sewage treatment plant was collected as a microbial source, and transported back to the laboratory under refrigeration. Prepare an inorganic medium containing 50mg / L estrogen as the sole carbon source, and inoculate it in a 500mL Erlenmeyer flask with 100mL of the above-mentioned inorganic medium according to the inoculum amount of 2.5% activated sludge. The inorganic component contained in the medium is K 2 HPO 4 , 0.1g / L; (NH 4 ) 2 HPO 4 , 0.1g / L; MgSO 4 ·7H 2 O, 0.02g / L; FeCl 3 , 0.01g / L; CaCl 2 2H 2O, 0.1g / L; NaCl, 0.1g / L. Use 2‰ acetic acid to adjust the pH value of the medium to 7.0-7.2, and sterilize it in an autoclave at 121°C for 20 minutes. After the temperature drops to about 30°C, inoculate it under the ultra-clean bench. After sludge inocul...

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Abstract

The invention relates to an oat acidovorax avenae strain capable of biodegrading natural estrogen and the application thereof, and the preservation code is CGMCC No. 3633; natural estrogen serves as the only one carbon source and is grown, can degrade estrone, 17Beta-estradiol and estriol with high efficiency, and can be used for a microbial technology for removing estrogen. The SS-1 strain can degrade the estrone with the initial concentration of 1mg / L by 99 percent within 12h, and degrade the 17Beta-estradiol and estriol with the initial concentration of 1mg / L by 99 percent within 24h. Estrone is generated in the degradation process of 17Beta-estradiol, and the estrone is also gradually degraded. Because the acidovorax avenae SS-1 strain has the property of effectively degrading the natural estrogen in the environment, and thereby can be applied in a technology for controlling wastewater and sewage which contain estrogen or a soil restoration technology.

Description

technical field [0001] The invention relates to an Acidovorax avenae bacterial strain capable of biodegrading natural estrogen and application thereof, in particular to using the Acidovorax avenae to effectively degrade estrone, 17β-estradiol and / or estriol. Background technique [0002] Estrone (Estrone, E1), 17β-Estradiol (17β-Estradiol, E2), and Estriol (E3) are natural estrogens, which are secreted by the ovaries of humans and animals, and will enter with feces and urine. Sewage treatment plants, soil, farmland and other environments. The synthetic estrogen 17α-ethinylestradiol (17α-Ethynylestradiol, EE2) is the main component of low-dose oral contraceptives, drugs for treatment of irregular menstruation and menopausal drugs, and will also be excreted into sewage treatment plants with feces and urine. figure 1 is the chemical structural formula of the above estrogen. The estrogen emitted by the livestock breeding industry will also be treated and disposed of through co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
Inventor 史江红宿凌燕曹金铃韩蕊
Owner BEIJING NORMAL UNIVERSITY
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