Method for culturing tissue blocks of mammary epithelial cells

Abstract

The invention discloses a method for culturing tissue blocks of mammary epithelial cells, which is characterized by comprising the following steps: 1) pretreatment: overspreading serum in a culture dish, and standing; 2) culture: culturing the tissue blocks in the culture dish, and collecting the mammary epithelial cells and fibroblasts; and 3) purification: removing the fibroblasts to obtain the mammary epithelial cells. The method saves a complicated step of preparing collagen in the traditional method, achieves high tissue block adherent rate and finally obtains a large number of mammary epithelial cells with good form.

Description

Method for planting tissue blocks of breast epithelial cells technical field The invention relates to a tissue block planting method of mammary gland epithelial cells. Background technique The mammary gland is one of the few organs in mammals that can repeatedly undergo the process of growth, functional differentiation and degeneration. Since foreign genes can be expressed in the mammary gland and then flow out with milk, mammary epithelial cells become important target cells in transgenic research. The in vitro culture of mammary epithelial cells is of great significance for studying the growth and development of mammary glands and elucidating the molecular regulation mechanism of lactation. It also provides an effective model for transgenic research. To culture mammary gland epithelial cells in vitro, a certain amount of primary mammary gland epithelial cells must be obtained first. Currently the most widely used method is the tissue block planting method. The tissue...

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Problems solved by technology

At present, homemade rat tail collagen is generally used in China, and the preparation process is very cumbersome. It needs to go through many steps such as soaking in ethanol, soaking in acid, 4 overnight and autoclaving.

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