Solid-phase synthesis of momordica charantia MC-JJ0102 polypeptide analogue under microwave irradiation and application thereof

Abstract

The invention relates to a momordica charantia MC-JJ0102 polypeptide analogue capable of regulating the blood sugar level and a microwave-irradiated solid phase synthesis method thereof. By modifying the site 2, 4, 5, 8 or 9 of natural momordica charantia polypeptide, the MC-JJ0102 polypeptide analogue with a longer time of pharmacological action is obtained; the chemical synthesis of the MC-JJ0102 polypeptide analogue can be efficiently and quickly carried out by the microwave-irradiated solid phase synthesis method, and crude product is preparatively and efficiently purified under the condition of liquid phase, and is finally lyophilized, so that the MC-JJ0102 polypeptide analogue is obtained.

Description

technical field [0001] The invention relates to a MC-JJ0102 polypeptide analog and a microwave-promoted solid-phase synthesis method thereof. Background technique [0002] Diabetes is an important disease that endangers human health today. At present, about 5%-7% of people in the world suffer from diabetes. It is considered to be the third largest killer of human disability and death after cardiovascular and cerebrovascular diseases and malignant tumors. . [0003] Diabetes is divided into type I and type II. Type I diabetes is an autoimmune disease that requires insulin injections for survival. Type II diabetes accounts for about 95% of the total number of diabetic patients. Type II diabetes is caused by metabolic disorders of islet cells, insufficient insulin secretion, or insulin resistance, which is a metabolic disease caused by insulin resistance mainly accompanied by relatively insufficient insulin secretion, or relatively insufficient insulin secretion accompanied b...

AI Technical Summary

Problems solved by technology

Using the traditional solid-phase method to synthesize long peptides, the yield is often very low and the impurities are more difficult to purify, thus losing the meaning of synthesis from a practical point of view

Secondly, the synthesis cycle is long. In the case of no difficult peptide sequence in the middle, it usually takes nearly a week to complete the synthesis cycle of 10 peptides. If there is a difficult peptide sequence, it is often impossible to synthesize the target peptide.