Frozen stock solution used for freezing and storing umbilical mesenchymal stem cells for long time

A technology of mesenchymal stem cells and cryopreservation solution, which is applied in the field of long-term cryopreservation of umbilical cord mesenchymal stem cells and its preparation, which can solve the problem that the activity of mesenchymal stem cells is short and unsuitable for long-term cryopreservation of mesenchymal stem cells And other issues

Inactive Publication Date: 2010-12-22
青岛奥克生物开发有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a mesenchymal stem cell cryopreservation medium, which does not contain fetal bovine serum, but is replaced by human serum albumin, but the time for preserving the activity of mesenchymal stem cells is short, and it is not suitable for long-term cryopreservation of mesenchymal stem cells

Method used

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  • Frozen stock solution used for freezing and storing umbilical mesenchymal stem cells for long time
  • Frozen stock solution used for freezing and storing umbilical mesenchymal stem cells for long time
  • Frozen stock solution used for freezing and storing umbilical mesenchymal stem cells for long time

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Embodiment Construction

[0016] 1. Preparation of cord plasma

[0017] (1) Put the umbilical cord blood containing 2.5% sodium citrate by mass (may not contain it, without affecting the technical effect of the present invention) in a 50ml sterile centrifuge tube, and centrifuge at 1000rpm for 15 minutes;

[0018] (2) Aspirate the supernatant, transfer it to a new centrifuge tube, centrifuge at 4000g for 20 minutes to remove platelets, and the supernatant is cord plasma for cryopreservation.

[0019] 2. Preparation of Mesenchymal Stem Cell Cryopreservation Solution

[0020] Liquid I: Take DMSO (dimethyl sulfoxide, purchased from SIGMA) and the above umbilical plasma, mix them uniformly in a volume ratio of 1:9, and place them at 4°C for later use;

[0021] Liquid II: Take DMSO (dimethyl sulfoxide) and fetal bovine serum (purchased from GIBCO) and mix them evenly at a volume ratio of 1:9, and place them at 4°C for later use.

[0022] 3. Cryopreservation of umbilical cord mesenchymal stem cells and detection of via...

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Abstract

The invention relates to a frozen stock solution used for freezing and storing umbilical mesenchymal stem cells for a long time, a preparation method and applications thereof, in particular to an umbilical mesenchymal stem cell. A new fetal calf serum substitute, namely the blood plasma from the umbilical blood is mainly adopted to prepare the frozen stock solution for freezing and storing the umbilical mesenchymal stem cells; the frozen stock solution can protect the umbilical mesenchymal stem cells from being injured in freezing for a long time; the vitality of the umbilical mesenchymal stem cell can achieve the same long-term freezing effect as the traditional frozen stock solution after the umbilical mesenchymal stem cell recovers, and avoid the umbilical mesenchymal stem cell from contacting with the heterogeneous animal source serum in the freezing and storing process, thus leading the clinical application thereof to be safer.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a freezing solution for long-term freezing of umbilical cord mesenchymal stem cells and a preparation method thereof. technical background [0002] Mesenchymal stem cells (MSCs) are a group of mesodermal-derived pluripotent stem cells with self-renewal and multidirectional differentiation potential. They are found in bone marrow, placenta, umbilical cord, amniotic membrane, muscle, fetal liver, cord blood and other tissues. It exists widely, because it has the advantages of convenient materials, simple and rapid expansion in vitro, and has broad application prospects in clinical treatment. Mesenchymal stem cells not only have the ability of multidirectional differentiation, but also support hematopoiesis and regulate immunity. Mesenchymal stem cells have been used in a large number of animal experiments and clinical trials at home and abroad. Mesenchymal stem cells have the ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 魏斯溧高宏王丽胡建霞张学峰张美荣
Owner 青岛奥克生物开发有限公司
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