A pharmaceutical composition containing pentoxifylline and diclofenac and its use
A technology of pentoxifylline and diclofenac, which is applied in the field of medicine, can solve the problems of pentoxifylline and diclofenac that have not yet been seen, and achieve the effects of reducing rectal vascular permeability, inhibiting swelling of the soles of the feet, and regulating activity
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Embodiment 1
[0026] Embodiment 1 rat adjuvant arthritis model experiment
[0027] experimental method:
[0028] 40 SD rats (about 200 grams) are randomly divided into four groups according to body weight, i.e. model control group, pentoxifylline group (0.3mg / kg), diclofenac sodium group (3.0mg / kg), composition group (containing pentoxifylline Ketoifylline 0.3mg / kg, diclofenac sodium 3.0mg / kg) in total 4 groups, 10 rats in each group.
[0029] Modeling of 4 groups of rats: intradermally injecting 0.1 mL of Freund's complete adjuvant into the plantar part of the right hind limb of the rats to establish a pathological model of adjuvant arthritis. On the 4th day after modeling, the rats in the pentoxifylline group, the diclofenac sodium group and the composition group were intragastrically administered once a day for a total of 21 days.
[0030] Before modeling, before administration, and 21 days after administration, the changes in the plantar circumference of the fixed part of the right hi...
Embodiment 2
[0048] The experiment of embodiment 2 rat croton oil hemorrhoid model
[0049] experimental method:
[0050] 50 SD rats (about 200 grams) were randomly divided into five groups according to body weight, i.e. model control group, pentoxifylline group (containing pentoxifylline 0.4%), diclofenac sodium group (containing diclofenac sodium 0.2%), composition group (containing pentoxifylline 0.4%, diclofenac sodium 0.2%), Ma Yinglong group, 10 rats in each group. Intrarectal administration to rats two days in advance, twice a day, soaked with 10mg cotton balls, stuffed the cotton balls into the rectum. should be removed). The last administration should be 2 hours after modeling.
[0051] The croton oil mixed liquid used for modeling the inflammatory agent was prepared with 1 part of distilled water, 4 parts of pyridine, 5 parts of ether and 10 parts of 15% croton oil ether solution. Cover the croton oil with 10 mg cotton ball, put the soaked cotton ball into the anus of the rat...
Embodiment 3
[0062] Example 3 Diclofenac Induced Gastric Mucosa Injury Experiment in Rats
[0063] experimental method:
[0064] 50 SD rats (about 200 grams) were randomly divided into five groups according to body weight, i.e. normal control group, diclofenac sodium group A (10mg / kg), diclofenac sodium group B (20mg / kg), composition A group (containing hexanone Theobromine 0.5 mg / kg, diclofenac sodium 10 mg / kg) and composition B group (containing pentoxifylline 0.5 mg / kg, diclofenac sodium 20 mg / kg) 10 rats in each group. Five hours after intragastric administration, aseptic operation was performed under ether anesthesia, 2-3 mL of blood was drawn from the heart, anticoagulated with heparin, the plasma was separated, stored at -20°C, and plasma TNF-α activity was detected. The stomach was quickly taken out, cut along the greater curvature, fixed on the platform and photographed, and the maximum diameter (DMAX) of each injury area was measured and integrated on the IBAS image analysis sys...
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