Legionella transport and enrichment medium
An enrichment medium and technology of Legionella, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of difficulty in isolation and culture, low positive rate of Legionella isolation and culture, etc. The positive rate of isolation and culture and the effect of reducing the inhibitory effect
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Embodiment 1
[0035] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:
[0036] Base fluid:
[0038] ACES 6,
[0039] a-ketoglutarate 0.5,
[0040] Iron pyrophosphate 0.2,
[0041] Amino acid protein solution:
[0042] Glycine 2.0,
[0043] L-cysteine 0.3,
[0044] Albumin 0.05,
[0045] Antibiotic solution:
[0046] Vancomycin 0.0005,
[0047] Polymyxin B 0.005,
[0048] Cycloheximide 0.005.
Embodiment 2
[0050] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:
[0051] Base fluid:
[0053] ACES 15,
[0054] a-ketoglutarate 1.5,
[0055] Iron pyrophosphate 0.3,
[0056] Amino acid protein solution:
[0057] Glycine 4.0,
[0058] L-cysteine 0.7,
[0059] Albumin 0.15,
[0060] Antibiotic solution:
[0061] Vancomycin 0.0015,
[0062] Polymyxin B 0.012,
[0063] Cycloheximide 0.012.
Embodiment 3
[0065] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:
[0066] Base fluid:
[0068] ACES 8-12,
[0069] a-ketoglutarate 0.8~1.2,
[0070] Iron pyrophosphate 0.24~0.28,
[0071] Amino acid protein solution:
[0072] Glycine 2.5~3.5,
[0073] L-cysteine 0.4~0.6,
[0074] Albumin 0.08~0.12,
[0075] Antibiotic solution:
[0076] Vancomycin 0.0008~0.0012,
[0077] Polymyxin B 0.008~0.010,
[0078] Cycloheximide 0.008~0.010.
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