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Method for promoting microalgae to synthesize tri-acylglycerol by using 2-methylacetoacetic acid ethyl ester

A technology of ethyl methyl acetoacetate and triacylglycerides, which is applied in the field of using ethyl 2-methyl acetoacetate to promote the synthesis of triacylglycerides by microalgae, which can solve the problem of occupying large cultivated land or forest land and ecological environment construction. and other issues to achieve the effect of increasing production and promoting the production of triacylglycerides

Inactive Publication Date: 2010-12-22
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the large-scale production of biodiesel from the above crops will occupy a large amount of arable land or forest land, which is inconsistent with the long-term development of human beings and the construction of the ecological environment.

Method used

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  • Method for promoting microalgae to synthesize tri-acylglycerol by using 2-methylacetoacetic acid ethyl ester
  • Method for promoting microalgae to synthesize tri-acylglycerol by using 2-methylacetoacetic acid ethyl ester

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Experimental program
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Effect test

Embodiment 1

[0013] This embodiment includes the following steps:

[0014] 1. Manually configure the medium, the composition of the medium is BG11 diluted 50%, and the total nitrogen and total phosphorus concentrations in the medium are controlled to be 15 mg·L respectively -1 and 1.5mg·L -1 ;

[0015] 2. Inoculate Scenedesmus into the artificially prepared medium, the preservation number of Scenedesmus is CGMCC3036, and the inoculation density is 3×10 5 mL -1 After the inoculation, add 0.5mg·L to the culture medium -1 Ethyl 2-methylacetoacetate EMA;

[0016] 3. Cultivate for 10 days, light-to-dark ratio 14h:10h, temperature 25°C, light intensity 1000-1400lux, centrifuge algae liquid to harvest algae cells; extract algae cell oil and triacylglyceride by organic solvent extraction.

[0017] refer to figure 1 , when no 2-methylacetoacetate EMA was added, the content of triacylglyceride TAG in the cell oil of Scenedesmus LX1 was 11.5%, adding 0.5mg·L -1 In the case of ethyl 2-methylace...

Embodiment 2

[0019] This embodiment includes the following steps:

[0020] 1. Manually configure the medium, the composition of the medium is BG11 diluted 50%, and the total nitrogen and total phosphorus concentrations in the medium are controlled to be 15 mg·L respectively -1 and 1.5mg·L -1 ;

[0021] 2. Inoculate Scenedesmus into the artificially prepared medium, the preservation number of Scenedesmus is CGMCC3036, and the inoculation density is 3×10 5 mL -1 After the inoculation, add 1.0mg·L to the culture medium -1 Ethyl 2-methylacetoacetate EMA;

[0022] 3. Cultivate for 10 days, light-to-dark ratio 14h:10h, temperature 25°C, light intensity 1000-1400lux, centrifuge algae liquid to harvest algae cells; extract algae cell oil and triacylglyceride by organic solvent extraction.

[0023] refer to figure 2 , when no 2-methylacetoacetate EMA was added, the triacylglyceride TAG content in the oil of Scenedesmus LX1 cells was 11.5%, adding 1.0mg·L -1 In the case of ethyl 2-methylacet...

Embodiment 3

[0025] This embodiment includes the following steps:

[0026] 1. Manually configure the medium, the composition of the medium is BG11 diluted 50%, and the total nitrogen and total phosphorus concentrations in the medium are controlled to be 10mg·L respectively -1 and 1.2mg·L -1 ;

[0027] 2. Inoculate Scenedesmus into the artificially prepared medium, the preservation number of Scenedesmus is CGMCC3036, and the inoculation density is 5×10 5 mL -1 After the inoculation, add 0.65mg·L to the culture medium -1 Ethyl 2-methylacetoacetate EMA;

[0028] 3. Cultivate for 15 days, light-to-dark ratio 12h:12h, temperature 20°C, light intensity 1000-1400 lux, centrifuge algae liquid to harvest algae cells; extract algae cell oil and triacylglycerides by organic solvent extraction

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Abstract

The invention provides a method for promoting microalgae to synthesize tri-acylglycerol by using 2-methylacetoacetic acid ethyl ester, which comprises the following steps: 1, preparing a culture medium artificially, wherein the components of the culture medium is BG11 which is diluted to 50%, and controlling the concentration of total nitrogen and total phosphorus in the culture medium to be respectively 10-15mg.L<-1> and 1.0-1.5mg.L<-1>; 2, inoculating Scenedesmus in the culture medium, wherein the inoculum density is (3-10)*105 Scenedesmus mL<-1>, after the inoculation is finished, adding 0.5-1.0mg.L<-1> of 2-methylacetoacetic acid ethyl ester EMA in the culture medium; and 3, culturing for 10-18 days, acquiring frustule from centrifuged algae solution, extracting frustule oil and tri-acylglycerol with an organic solvent extraction process. The added 2-methylacetoacetic acid ethyl ester in the invention can promote the content of the tri-acylglycerol in the Scenedesmus frustule and the output of the tri-acylglycerol in unit algae solution, thereby improving the output of the biodiesel raw materials and the application potential for prepare the biodiesel by using the microalgae.

Description

technical field [0001] The invention belongs to the technical field of new biological energy development, and in particular relates to a method for promoting microalgae to synthesize triacylglycerides by utilizing ethyl 2-methylacetoacetate. Background technique [0002] In recent years, worldwide population growth and industrial development have brought about excessive consumption and exhaustion of resources and energy; the energy crisis is the biggest challenge facing mankind in the 21st century. The only way to meet this challenge is to develop sustainable, renewable and environmentally friendly new energy sources. Biodiesel is a new type of bio-new energy that has received widespread attention. A relatively mature technology has been developed in the preparation of biodiesel from grains, oil crops or lignin crops and has market applications. However, the large-scale production of biodiesel from the above crops will occupy a large amount of arable land or forest land, w...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/13Y02E50/10
Inventor 胡洪营李鑫
Owner TSINGHUA UNIV
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