Composite bacterial agent for methane fermentation
A compound bacterial agent and biogas fermentation technology, applied in the field of microorganisms, can solve the problems of strong gas production capacity, inability to apply large-scale fermentation projects, and short start-up time of bacterial agent fermentation, etc., to achieve the effect of optimal combination
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specific Embodiment approach 1
[0013] Specific embodiment one: The composite microbial agent for biogas fermentation of the present embodiment consists of 8 parts of Bacteroides cellulosolvens fermentation broth and 12 parts of Clostridium pasteurianum fermentation broth in parts by weight , 30 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation liquid and 20 parts of Methanosarcina mazei (Methanosarcina mazei) fermentation liquid; the effective number of viable bacteria in the fermentation liquid of the above four kinds of bacteria is 10 9 Individuals / mL or more.
[0014] The steps of the preparation method of this composite bacterial agent are as follows:
[0015] (1) Activation of strains: Preserved Bacteroides cellulosolvens, Clostridium pasteurianum, Methanoculleus marisnigri and Methanosarcina mazei were inoculated into anaerobic tubes containing activation medium, and then inoculated with 6×10 -2 After the vacuum pump of pa vacuumizes, fill the nitrogen gas with a ...
specific Embodiment approach 2
[0019] Specific embodiment two: The composite bacteria agent for biogas fermentation of the present embodiment consists of 15 parts of Bacteroides cellulosolvens fermentation broth and 20 parts of Clostridium pasteurianum fermentation broth in parts by weight , 45 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation broth and 20-50 parts of Mazei Methanosarcina (Methanosarcina mazei) fermentation broth; the effective number of viable bacteria in the fermentation broth of the above four kinds of bacteria is 10 9 Individuals / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.
[0020] Inoculate the composite bacterial agent of this embodiment in a biogas fermenter with cow dung with a TS (total solid concentration) of 8-10% as the main raw material, perform anaerobic fermentation at 35°C, and the peak of the daily gas production rate occurs on the fifth day , with a peak val...
specific Embodiment approach 3
[0021] Specific embodiment three: The composite microbial agent for biogas fermentation of the present embodiment consists of 11 parts of Bacteroides cellulosolvens fermentation broth and 16 parts of Clostridium pasteurianum fermentation broth in parts by weight , 38 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation liquid and 35 parts of Methanosarcina mazei (Methanosarcina mazei) fermentation liquid composition; The number of effective viable bacteria in the fermentation liquid of above four kinds of bacteria is 10 9 Individuals / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.
[0022] Inoculate the composite bacterial agent of this embodiment in a biogas fermenter with cow dung with a TS (total solid concentration) of 8-10% as the main raw material, perform anaerobic fermentation at 35°C, and the peak of the daily gas production rate occurs on the fourth day , wit...
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