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Composite bacterial agent for methane fermentation

A compound bacterial agent and biogas fermentation technology, applied in the field of microorganisms, can solve the problems of strong gas production capacity, inability to apply large-scale fermentation projects, and short start-up time of bacterial agent fermentation, etc., to achieve the effect of optimal combination

Active Publication Date: 2011-12-14
黑龙江省昊千生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation temperature of large and medium-sized biogas projects is mostly a constant temperature, and the fermentation start-up time of the bacterial agent is short and the gas production capacity is strong. However, most of the various microbial agents used for biogas fermentation in the existing reports are only suitable for farmers. small-scale biogas fermentation tanks, but cannot be applied to large-scale fermentation projects
At present, there is no report on the fermenting agent suitable for large and medium-sized biogas projects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific embodiment one: The composite microbial agent for biogas fermentation of the present embodiment consists of 8 parts of Bacteroides cellulosolvens fermentation broth and 12 parts of Clostridium pasteurianum fermentation broth in parts by weight , 30 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation liquid and 20 parts of Methanosarcina mazei (Methanosarcina mazei) fermentation liquid; the effective number of viable bacteria in the fermentation liquid of the above four kinds of bacteria is 10 9 Individuals / mL or more.

[0014] The steps of the preparation method of this composite bacterial agent are as follows:

[0015] (1) Activation of strains: Preserved Bacteroides cellulosolvens, Clostridium pasteurianum, Methanoculleus marisnigri and Methanosarcina mazei were inoculated into anaerobic tubes containing activation medium, and then inoculated with 6×10 -2 After the vacuum pump of pa vacuumizes, fill the nitrogen gas with a ...

specific Embodiment approach 2

[0019] Specific embodiment two: The composite bacteria agent for biogas fermentation of the present embodiment consists of 15 parts of Bacteroides cellulosolvens fermentation broth and 20 parts of Clostridium pasteurianum fermentation broth in parts by weight , 45 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation broth and 20-50 parts of Mazei Methanosarcina (Methanosarcina mazei) fermentation broth; the effective number of viable bacteria in the fermentation broth of the above four kinds of bacteria is 10 9 Individuals / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.

[0020] Inoculate the composite bacterial agent of this embodiment in a biogas fermenter with cow dung with a TS (total solid concentration) of 8-10% as the main raw material, perform anaerobic fermentation at 35°C, and the peak of the daily gas production rate occurs on the fifth day , with a peak val...

specific Embodiment approach 3

[0021] Specific embodiment three: The composite microbial agent for biogas fermentation of the present embodiment consists of 11 parts of Bacteroides cellulosolvens fermentation broth and 16 parts of Clostridium pasteurianum fermentation broth in parts by weight , 38 parts of Black Sea methane bag-shaped fungus (Methanoculleus marisnigri) fermentation liquid and 35 parts of Methanosarcina mazei (Methanosarcina mazei) fermentation liquid composition; The number of effective viable bacteria in the fermentation liquid of above four kinds of bacteria is 10 9 Individuals / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.

[0022] Inoculate the composite bacterial agent of this embodiment in a biogas fermenter with cow dung with a TS (total solid concentration) of 8-10% as the main raw material, perform anaerobic fermentation at 35°C, and the peak of the daily gas production rate occurs on the fourth day , wit...

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Abstract

The invention discloses a composite bacterial agent for methane fermentation, which relates to a composite preparation of microbial fermentation bacterial liquid. In the invention, the problem of lacking bacterial gent applicable to methane fermentation using cow dung as a main raw material and bacterial agent applicable to medium and large methane fermentation projects is solved. The composite bacterial agent comprises 8 to 15 weight parts of bacteroides cellulosolvens fermentation liquor, 12 to 20 weight parts of clostridium pasteurianum fermentation liquor, 30 to 45 weight parts of methanoculleussubmarinua fermentation liquor and 20 to 50 weight parts of methanosarcina mazei fermentation liquor. The number of the active bacteria of 1 milliliter of the fermentation liquor of each of the four kinds of bacteria is greater than 10<9>. The composite bacterial agent for the methane fermentation using the cow dung as the main raw material can make gas production earlier and improve gas yield and methane yield effectively.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a compound preparation of microbial fermentation broth. Background technique [0002] Biomass energy has always been an important source of energy for human survival, ranking fourth in the world's total energy consumption after coal, oil and natural gas. Biogas plays an important role in the field of biomass energy, and biogas fermentation technology is also one of the most important technologies in biomass energy conversion. Biogas fermentation, also known as anaerobic digestion and anaerobic fermentation, refers to the catabolism of organic substances by various microorganisms under certain moisture, temperature and anaerobic conditions, and finally forms flammable mixed gases such as methane and carbon dioxide (biogas) the process of. The organic matter that can be used for biogas fermentation usually includes crop straw, weeds, animal manure, food residue, pulp, mu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C02F11/02C12R1/01C12R1/145
Inventor 王彦杰王伟东戚桂娜高亚梅张波荆瑞勇
Owner 黑龙江省昊千生物科技有限公司
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