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Method and detection strip for rapidly detecting human blood group antibodies

A blood group antibody and human detection technology, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of large influence of subjective operation deviation of test results, inapplicability of fast and portable detection, and high manual labor intensity. Reduced complexity and cost of reagents and assays, long shelf life, and ease of interpretation

Inactive Publication Date: 2011-01-26
INTEC PROD INC
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Problems solved by technology

[0007] In short, the currently commonly used anti-typing identification methods have problems such as short validity period, high manual labor intensity, high cost, test results are greatly affected by subjective operation deviation, and the possibility of contamination.
Moreover, the traditional anti-typing detection reagent is still not suitable for the needs of fast and portable detection, therefore, it is necessary to make substantial improvements to the traditional anti-typing blood group detection method

Method used

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  • Method and detection strip for rapidly detecting human blood group antibodies
  • Method and detection strip for rapidly detecting human blood group antibodies

Examples

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Embodiment 1

[0043] Example 1: Preparation and use of a test strip for detecting human ABO blood group antibodies

[0044] 1) Extraction of blood group antigen: red blood cells were swollen with 10 mM phosphate buffer containing 1% sodium dodecylsulfate, and treated with organic solvent ethanol (70° C., 30 minutes). After removing the protein, the glycolipids are collected and separated by affinity chromatography to obtain the desired type A and type B carbohydrate blood group antigen extracts.

[0045] 2) The blood group antigen A / B is directly or indirectly fixed on the A zone of the nitrocellulose membrane 1, and the corresponding anti-blood group antibody (anti-A / anti-B) is fixed on the B zone of the nitrocellulose membrane ( see figure 1 ); the immobilization method used has little effect on the biological activity of the antigen / antibody, and sucrose is added to the immobilized antigen / antibody as a protein preservative.

[0046] 3) After the blood group antigen A / B is coupled with...

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Abstract

The invention relates to a method for the immunodetection of human blood group antibodies, particularly relating to a method and a used detection strip for rapidly detecting human blood group antibodies. The method comprises the following steps: a blood group antigen is precoated on a solid-phase carrier; the blood group antigen with a signal mark is fixed on a fiber mat adhered on one end of the carrier; and a sample is added dropwise, if the sample to be detected contains an antibody which can be combined with the blood group antigen, a macroscopic immune complex can be observed on the solid-phase carrier, and otherwise, the macroscopic immune complex is not formed, thus the blood group antibody in the sample is detected.

Description

field of invention [0001] The invention relates to a method for immunological detection of human blood group antibodies, in particular to a reverse typing detection method for rapid detection of human blood group, and a detection strip used for completing the method. Background of the invention [0002] The principle of blood typing is based on the agglutination of cells produced by antigen-antibody reactions. The use of blood typing reagents to detect erythrocyte surface antigens is called the positive typing method; on the contrary, the use of erythrocyte surface antigens to detect the corresponding antibodies in the serum is called the reverse typing method. In order to avoid secondary hemolytic transfusion reactions caused by blood type incompatibility, it is necessary to detect red blood cell surface antigens and antibodies in serum or plasma to ensure the safety of clinical blood transfusion. The blood type monitoring standards of healthy blood donors published in the...

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Application Information

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IPC IPC(8): G01N33/551G01N33/558G01N33/532G01N33/80
Inventor 汪大明林永志王保丹周汉博
Owner INTEC PROD INC
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