Immunoglobulin (IgY) antibody oral spray for preventing decayed teeth

An oral spray, antibody technology, applied in the direction of antibody, aerosol delivery, digestive system, etc., can solve the problems of reduced antibacterial activity, reduced titer, disappearance, etc., to prevent dental caries, eliminate dental plaque, and prevent dental caries. Effect

Active Publication Date: 2011-04-06
赵凯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the IgY antibody is unstable, IgY will be degraded and inactivated, the titer of IgY will decrease, and the antibacterial activity of IgY will decrease or even disappear

Method used

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  • Immunoglobulin (IgY) antibody oral spray for preventing decayed teeth
  • Immunoglobulin (IgY) antibody oral spray for preventing decayed teeth
  • Immunoglobulin (IgY) antibody oral spray for preventing decayed teeth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation and Extraction of IgY Antibody

[0044] Using BHI or TTY medium, culture c-type and d-type Streptococcus mutans (Shanghai Jiaotong University Institute of Stomatology) for 48 hours under the temperature condition of 37 ℃, respectively, the cultured c-type and d-type streptococcus mutans were respectively used at 5000rpm Centrifuge for 5 minutes, collect the bacteria, and wash the bacteria three times with a phosphate buffer solution with a pH value of 6.0 and a concentration of 0.2M. Heat treatment at 50°C for 25 minutes to prepare c-type Streptococcus mutans (2 billion / ml) and d-type Streptococcus mutans (2 billion / ml) liquids, mix them in a volume ratio of 5:1, and prepare immunogens.

[0045] Take 1.0ml of the antigen each time, inject it subcutaneously to laying hens (Leghorn chickens), and immunize three times with an interval of two weeks.

[0046] Collect the eggs 20 days after the first immunized hen, separate the egg yolk with an egg yolk...

Embodiment 2

[0048] 1) Formulation:

[0049]

[0050] 2) Preparation: add the above components into the container respectively, inactivate the virus by Pasteur sterilization, filter and sterilize with a 0.22 μm membrane, and obtain the anti-caries IgY antibody oral spray of the present invention. Then, pack it into a small carry-on spray can and spray it.

[0051] 3) In vitro antibacterial test: Take 200 μl of the anti-caries IgY antibody oral spray prepared in 2), and test it according to the above in vitro antibacterial test. After testing: the bacteriostatic rate to Streptococcus mutans type C is 80%, and the bacteriostatic rate to Streptococcus mutans Type D is 85%.

example 3

[0053] 1) Formulation:

[0054]

[0055]

[0056] 2) preparation: with embodiment 2.

[0057] 3) In vitro antibacterial test: Take 200 μl of the anti-caries IgY antibody oral spray prepared in 2), and test it according to the above in vitro antibacterial test. After testing: the bacteriostatic rate to Streptococcus mutans type C is 80%, and the bacteriostatic rate to Streptococcus mutans Type D is 85%.

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Abstract

The invention discloses an immunoglobulin (IgY) antibody oral spray for preventing decayed teeth. The spray comprises the following components in percentage by weight: 0.1 to 0.5 percent of IgY, 5 to 15 percent of bacteriostat, 3 to 6 percent of preservative, 5 to 20 percent of glycerin, 5 to 10 percent of ethanol, 0.1 to 1 percent of edible essence and 50 to 75 percent of distilled water. The IgY antibody oral spray for preventing the decayed teeth is convenient to manufacture and use, can effectively prevent the decayed teeth and treat gingivitis, periodontitis and canker sore, and has high safety, the bacteriostatic ratio to streptococcus mutans c is over 80 percent, and the bacteriostatic ratio to streptococcus mutans d is over 85 percent. In addition, the IgY antibody oral spray for preventing the decayed teeth can be stably preserved at normal temperature for at least three years.

Description

technical field [0001] The invention relates to the field of oral health care, in particular to an IgY antibody oral spray that can prevent dental caries, remove dental plaque, treat gingivitis, periodontitis, oral ulcers and eliminate bad breath. Background technique [0002] Dental caries (commonly known as tooth decay) refers to a disease in which tooth tissue is gradually destroyed and disintegrated to form a defect. Caries is the most common dental disease in humans. The prevalence rate is generally about 70%, and in some places it is as high as more than 90%. The World Health Organization lists it, together with cancer and cardiovascular disease, as the three major non-communicable diseases that should be prevented and controlled. Dental caries not only causes tooth collapse and defect, but also causes diseases of the apex of the pulp tissue and surrounding tissues, and even causes complications such as bone marrow inflammation caused by the infection of the pulp bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K9/12A61P1/02
Inventor 赵凯
Owner 赵凯
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