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Method for cleaning inner wall of pipeline type photobioreactor pipe

A photobioreactor and cleaning method technology, applied in the field of microalgae biology, can solve the problems of rough surface of the reactor material, increase of the number of dead corners of the photobioreactor, and collapse of the culture system, so as to reduce the difficulty of manual cleaning and be applied in large-scale production Effects of value, production and application economy

Active Publication Date: 2012-07-25
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0011] Once the microalgae cells adhere to the wall, a vicious cycle will occur and the following series of adverse effects will be formed, which will make the cell culture effect continue to decline, and even lead to the collapse of the entire culture system
First of all, after the microalgae cells adhere to the inner wall of the reactor, the surface of the reactor material is rough, which increases the fluid resistance, reduces the flow rate of the algae solution, and further increases the number of dead spots in the photobioreactor, making it easier for the cells to adhere. wall phenomenon, a vicious cycle in which the degree of cell attachment is continuously aggravated; secondly, after the microalgae cells are attached to the wall, the formed microalgae spots (or thin layers of microalgae) are also light-isolated spots (or even light-isolated layers), varying degrees It affects the light permeability of the photobioreactor and hinders the light from entering the photobioreactor, so the light absorbed by the internal microalgae cells is insufficient, and the photosynthetic efficiency of the microalgae cells decreases; thirdly, the adherent cells near the pipeline are always under strong light state, the light is oversaturated and light damage occurs, resulting in a decrease in the photosynthetic efficiency of this part of the cell, and even cell death
In addition, if the adherent cells die, the algae will rot, which will also affect the quality of microalgae products.

Method used

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  • Method for cleaning inner wall of pipeline type photobioreactor pipe

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Embodiment

[0056] Embodiment: the cleaning technology method of pipeline photobioreactor of 5 tons scale

[0057] First, a set of pipeline photobioreactors was constructed. The transparent material uses glass tubes with a length of 200cm and an inner diameter of 5.4cm; first, 25 glass tubes are connected in series to form a pipe with a length of 5000cm; 18 pipes; the pipes arranged on both sides of the support frame are respectively connected together by elbows. The above-mentioned light-receiving pipeline part, together with the gas exchange and temperature control tower, etc. form a pipeline photobioreactor with a volume of 5 tons.

[0058] If conventional technology is adopted, after the pipeline photobioreactor is inoculated with microalgae (Haematococcus motile cells) and cultivated for 7 days, a large amount of attachments will appear on the tube wall (sampling microscopic examination is mainly caused by the non-transformation of Haematococcus motile cells). Kinetic cells), no ma...

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Abstract

The invention belongs to the biologic field of micro algae, relating to a method for cleaning inner wall of pipeline type photobioreactor pipe in micro algae culture. Magnetic sphere is placed into a pipeline type photobioreactor, culture solution flows when being pushed by power, the magnetic sphere randomly enters into the pipeline of photobioreactor along with the culture solution and does directional movement or slides under manual regulation of a control system outside the pipeline, and the magnetic sphere continuously slightly collides and rubs pipe wall in circulated moving process in the photobioreactor pipeline, so that algae cell material adhered onto transparent pipeline inner wall is rubbed into liquid phase used for culturing micro algae, the pipeline photobioreactor pipelineis effectively cleaned and light transmission thereof is maintained. The invention can effectively clean cells into algae liquid phase at initial stage of adherence, or substance such as cell adheredto the inner wall of the pipeline type photobioreactor pipe is removed, restriction of micro algae cell adherence to micro algae cell culture is overcome, and good light transmission and culture effect of photobioreactor are maintained.

Description

technical field [0001] The invention belongs to the field of microalgae biology and relates to a method for cleaning the inner wall of a pipe photobioreactor for microalgae cultivation. Background technique [0002] As an important part of primary productivity in the biosphere, microalgae are the only bait for the entire growth process (or specific developmental stages) of aquatic animals in the aquatic ecosystem or the bait for bait organisms. At the same time, microalgae are close to 50% of plant species, and have diversity, complexity and particularity in terms of germplasm, ecological distribution, genetic information, biochemical composition, and metabolic pathways, which further determine their potential nutritional and pharmaceutical value. Humans may develop a large number of structurally specific high value-added bioactive ingredients from them. [0003] Energy is not only the lifeblood of economic development but also the driving force of social development, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B08B9/027
Inventor 刘建国袁毅李凌黄园林伟
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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