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Method for obtaining high-efficiency regeneration plant of ephemeral plant lachnoloma growing in early spring

A plant and short-lived technology, applied in the field of plant tissue culture and plant biology, can solve the problems of low natural reproduction rate, extinction threat, and shortened growth and development cycle of A. big effect

Inactive Publication Date: 2011-04-13
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the blank technical status of ephemeral plants in tissue culture research, the present invention aims to provide a method for establishing a high-efficiency regeneration system of early spring ephemeral plant Aquinas chinensis, which fills up the technical gaps in tissue culture of ephemeral plants in the prior art and solves the problem The low natural reproduction rate of Camellia mellifera is facing the threat of extinction. The growth and development cycle is shortened, and the in vitro regenerated plants under artificial culture conditions and culture medium are obtained.

Method used

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  • Method for obtaining high-efficiency regeneration plant of ephemeral plant lachnoloma growing in early spring
  • Method for obtaining high-efficiency regeneration plant of ephemeral plant lachnoloma growing in early spring
  • Method for obtaining high-efficiency regeneration plant of ephemeral plant lachnoloma growing in early spring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment one: standby of aseptic material

[0028] Culture medium: MS (Murashige and Skoog, 1962) medium was prepared by selecting 10×macroelement mother liquor, 100×iron salt mother liquor, 100×trace element mother liquor, and 100×organic matter (vitamin) mother liquor, without adding any growth regulator The culture medium contains 3% sucrose and 0.6% agar powder, and the pH value is adjusted to 6.0. It is divided into 150mL Erlenmeyer flasks, and each bottle is about 50mL. Put it into a sterilizing pot after subpackaging, at 1.2×104kg / m 2 (121°C) for 20 minutes of sterilization, take it out, put it flat, and cool it for later use.

[0029]Cultivation of sterile seedlings: The mature Mianguo mustard fruit was collected in Cainan, Fukang, Xinjiang. After the fruit is picked, store it at room temperature for later use. Take the seeds that are large and plump and free from diseases and insect pests, wash them with tap water for 2-3 minutes, then soak them in 70% eth...

Embodiment 2

[0030] Embodiment two: the induction of callus

[0031] This test is for the purpose of establishing a best system for callus induction of A. sativa. The hypocotyl and cotyledon of A. sativa are used as explants, and different plant growth regulators (IAA, 2, 4-D, NAA, IBA, BAP) in different concentrations and combinations of its callus induction and growth were compared.

[0032] 1. Screening of Different Explants and Different Growth Regulators in Callus Induction

[0033] 14-15 days after the germination of mustard seeds, the hypocotyls and cotyledons of the aseptic seedlings were cut into 0.5 cm long, 0.5 × 0.3 cm 2 area, and inoculate the MS medium of adding different plant growth regulators (IAA, 2,4-D, NAA, IBA, BAP) in the prior art and the MS basic medium without adding any phytohormone as a control (CK), See Table 1. All media contain 3% sucrose and 0.6% agar powder, the pH value is adjusted to 6.0, at 1.2×104kg / m 2 (121°C) for 20 minutes (Shirin et al., 2007). ...

Embodiment 3

[0050] Embodiment three: the proliferation of callus

[0051] This test is for the purpose of establishing a best system for the callus proliferation of A. sativa, and the hypocotyl and cotyledon of A. sativa are used as explants, and different plant growth regulators (IAA, 2, 4-D, NAA, IBA, BAP) in different concentrations and combinations of its effects on callus proliferation were compared.

[0052] 1. Method for Callus Proliferation

[0053] The hypocotyl and cotyledon callus induced in the above examples were cut into 0.5×0.5cm 2 Subculture in the subculture medium supplemented with different phytohormones, see Table 2. The concentration of sucrose and agar powder added to the medium and the culture conditions are the same as those in 2.1.1.

[0054] Table 2. Different concentrations of auxin and cytokinin and their combinations

[0055]

[0056]

[0057] The above experiments show that the callus induced by cotyledons has a better proliferation efficiency than ...

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Abstract

The invention discloses a method for establishing a regeneration system of ephemeral plant lachnoloma growing in early spring, comprising the following steps of: comparing the influence of different explants, a growth regulating agent, sugar concentration and culture conditions on the induction on callus and multiplication of the callus, the differentiation of buds and the root induction with the influence of pretreatment of different matrixes and regeneration seedlings on the transplanting survival rate, determining the explants using the callus as cotyledon; compatibly setting a growth regulating agent to be MS+1.0mg / l IBA+0.1mg / l BAP; carrying out tissue culture under the dark condition with the concentration of sucrose being 3 percent or 4 percent; and establishing an optimal system of the induction of the callus of the lachnoloma and the plant regeneration, thereby obtaining the high-efficiency regeneration plant of the ephemeral plant lachnoloma growing in early spring. The invention solves the problem that the lachnoloma is threatened with extinction due to low natural breeding rate, better shortens the growth and development cycle, can be used for obtaining an in vitro regeneration plant under the real artificial culture condition and the culture medium and has wide application value.

Description

field of invention [0001] The invention relates to the field of plant biotechnology. Specifically, the present invention relates to the technical field of plant tissue culture. Background technique [0002] Ephemeral plants are a general term for a special plant group with a short life cycle or annual growth period that grows in arid desert regions (Risser and Cottam, 1968; Mulroy and Rundel, 1977; Zhang Liyun, 1985; Huang Peihu, 2002). Mainly distributed in the desert areas of Central Asia, West Asia, North Africa, North America, South America and the Mediterranean coast (Went, 1948; Shreve, 1951; Alekhin, 1954; Senikoff, 1954; Rooyen et al., 1992; Telenius, 1993; Hegazy and Elamry, 1998; Wilby and Shachak, 2000; Gutterman, 2001), Central Asia is one of its distribution centers (Mao Zumei and Zhang Dianmin, 1994; Huang Peihu, 2002). The ephemeral plants in my country are only distributed in the deserts in northern Xinjiang (especially the Junggar Desert) and their adjacen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 廖康谭敦炎满苏尔
Owner XINJIANG AGRI UNIV